Objective:To establish mice model with learning and memory deficit induced by cerebral ischemia/reperfusion.Methods:The mice model induced by cerebral ischemia/reperfusion were made by bilateral occlusion of both common carotid arteries and ischemic hypotension/reperfusion.Locomotive activity

step-down test and water maze were used to reflect behavior changes

as well as cell morphologic changes were evaluated

respectively.And total anti-oxidant capacity and activities of SOD(superoxide dismutase)and MDA(malondialdehyde)were also measured.Results:The neuronal damage in model mice was significant

compared with sham group.And locomotive activity of model mice markedly decreased.The escape latency and time of electric shock of model mice significantly increased in step-down test;and 24 hours later the number of errors significantly increased

step-down latency and time of remaining on platform markedly decreased.In maze test

the spatial learning and memory ability of model mice significantly decreased with characteristics of increase in the number of errors and latency at D3

D4 and D5

and the total anti-oxidant capacity and activities of SOD were decreased and MDA level increased in model group compared with sham group

respectively.Conclusion:This model of mice cerebral ischemia/reperfusion is useful for studying learning and memory deficit.