Influence on humoral immunity in mice by goat placenta immunoregulatory factor

FANG Liaoqiong; et al

doi:10.13406/j.cnki.cyxb.2005.03.013

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Influence on humoral immunity in mice by goat placenta immunoregulatory factor

更新时间：2024-01-17

- Influence on humoral immunity in mice by goat placenta immunoregulatory factor
- Journal of Chongqing Medical University Issue 3, Pages: 356-358(2005)
- 作者机构：
  
  1. 第三军医大学实验动物教研室
  2. 西南师范大学生命科学院
  3. 新疆农业大学动物医学院
  4. 第三军医大学实验动物教研室,重庆,400038
  5. 现单位为西南农业大学蚕学与生物技术学院
  6. ,重庆,400038
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2005.03.013
  CLC： S852.4
- Published：2005，
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[1]方廖琼,钟英英,张华,牛荣,魏泓.羊胎盘免疫调节因子对小鼠体液免疫的影响[J].重庆医科大学学报,2005(03):356-358.

FANG Liaoqiong, et al. Influence on humoral immunity in mice by goat placenta immunoregulatory factor. [J]. Journal of Chongqing Medical University (3):356-358(2005)
[1]方廖琼,钟英英,张华,牛荣,魏泓.羊胎盘免疫调节因子对小鼠体液免疫的影响[J].重庆医科大学学报,2005(03):356-358. DOI： 10.13406/j.cnki.cyxb.2005.03.013.

FANG Liaoqiong, et al. Influence on humoral immunity in mice by goat placenta immunoregulatory factor. [J]. Journal of Chongqing Medical University (3):356-358(2005) DOI： 10.13406/j.cnki.cyxb.2005.03.013.

摘要

目的:探讨羊胎盘免疫调节因子对机体的体液免疫调节作用。方法:BALB/c小鼠为实验对象

MTT法测定脾B淋巴细胞的增殖

溶血空斑实验（PFC）测定IgM

酶联免疫吸附法（ELISA）测定体外培养B淋巴细胞培养上清IgG、体内血清抗体IgG。结果:羊胎盘免疫调节因子对免疫抑制小鼠分泌抗体能力具有显著改善（P<0.01）

并具有剂量效应（P<0.01）;直接作用于脾淋巴细胞

可显著促进细菌脂多糖诱导的小鼠脾淋巴细胞增殖

显著提高脾B淋巴细胞产生抗体的能力（P<0.01）

剂量效应不显著。结论:羊胎盘免疫调节因子能增强小鼠体液免疫功能

可促进免疫抑制小鼠的体液免疫能力恢复。

Abstract

Objective:To study the humoral immunity effect of goat placenta immunoregulatory factor(GPIF).Methods:Basing on BALB/c mice

the study was carried out by determining the proliferation of B lymphocyte in mice spleen with MTT method

the level of IgM with PFC test

and the level of IgG secreted by mice B cell in vivo and in vitro with ELISA test.Results:GPIF could effeciently increase the level of antibody in vivo.Direct experiment on splenic B lymphocyte

GPIF could also improve the level of antibody in vitro and enable mice lymphocyte induced by LPS to proliferate efficiently.Conclusion:GPIF conld enhance humoral immunity function and recover the ability of humoral immunity in mice with immunodeficiency.

关键词

羊胎盘免疫调节因子体液免疫B淋巴细胞

Keywords

Goat placenta immunoregulatory factor(GPIF)humoral immunityB lymphocyte

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