Effects of curcumin on proliferation in human ovary cancer cell line SKOV<sub>3</sub>

CHEN Xiaofen; et al

doi:10.13406/j.cnki.cyxb.2005.02.026

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Effects of curcumin on proliferation in human ovary cancer cell line SKOV₃

更新时间：2024-01-17

- Effects of curcumin on proliferation in human ovary cancer cell line SKOV₃
- Journal of Chongqing Medical University Issue 2, Pages: 256-259(2005)
- 作者机构：
  
  1. 重庆医科大学
  2. 重庆医科大学病理生理教研室
  3. 重庆医科大学病理生理教研室 97级七年制
  4. ,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2005.02.026
  CLC： R737.31
- Published：2005，
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[1]陈小芬,汤为学,李龙江.姜黄素对人卵巢癌细胞株SKOV_3的抗增殖作用[J].重庆医科大学学报,2005(02):256-259.

CHEN Xiaofen, et al. Effects of curcumin on proliferation in human ovary cancer cell line SKOV₃. [J]. Journal of Chongqing Medical University (2):256-259(2005)
[1]陈小芬,汤为学,李龙江.姜黄素对人卵巢癌细胞株SKOV_3的抗增殖作用[J].重庆医科大学学报,2005(02):256-259. DOI： 10.13406/j.cnki.cyxb.2005.02.026.

CHEN Xiaofen, et al. Effects of curcumin on proliferation in human ovary cancer cell line SKOV₃. [J]. Journal of Chongqing Medical University (2):256-259(2005) DOI： 10.13406/j.cnki.cyxb.2005.02.026.

摘要

目的:探讨姜黄素对人卵巢癌细胞株SKOV3 的抑制增殖作用及其机制。方法:采用MTT法测定姜黄素在不同浓度、不同时间对SKOV3 的抑制增殖效应

流式细胞术分析细胞周期分布及凋亡率

透射电镜观察细胞的超微结构变化

免疫细胞化学法检测凋亡相关基因蛋白的表达。结果:姜黄素可抑制SKOV3 细胞的生长

其抑制率与药物浓度和作用时间呈依赖关系

72h的中效浓度（IC50 ）为79.96 μmol/L

流式细胞仪分析证实姜黄素能使SKOV3 细胞聚积在G2 /M

电镜观察发现姜黄素可诱导细胞凋亡

姜黄素作用72h后

免疫细胞化学表明突变型P5 3蛋白（MTP5 3）及Bcl- 2表达减弱

Bax及Fas表达增强。结论:姜黄素对SKOV3 细胞有抑制增殖作用

通过下调MTP5 3、Bcl- 2的表达及上调Bax、Fas的表达而诱导细胞凋亡

Abstract

Objective:To investigate the proliferation inhibitory effect of curcumin on human ovary cancer cell line SKOV 3 and the mechanisms.Methods:The MTT method was used to assay the biologic activities of curcumin at different time and in different doses.The cell cycle distribution was detected by flow cytometric analysis.The cell ultrastructure was observed by electron microscope.The expression of apoptosis-associated genes protein was detected with immunohistochemical technique.Results:Curcumin could effectively inhibit SKOV 3 in a dose- and time-dependent manner.IC 50 of curcumin to SKOV 3 was 79.96μmol/L in 72 hours.The cell growth was arrested at G 2/M stage.Curcumin could lead to apoptosis.When treated with curcumin for 72 hours

the expression of MTP53 and Bcl-2 were down-regulated

while the expression of Bax and Fas were up-regulated.Conclusion:Curcumin can inhibit the proliferation of SKOV 3.The apoptosis is related to the down-regulation of MTP53 and Bcl-2 and up-regulation of Bax and Fas.

关键词

姜黄素卵巢癌增殖凋亡

Keywords

curcuminovarian cancerproliferationapoptosis

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Effects of curcumin on proliferation in human ovary cancer cell line SKOV3

DOI：10.13406/j.cnki.cyxb.2005.02.026

摘要

Abstract

关键词

Keywords

references

Effects of curcumin on proliferation in human ovary cancer cell line SKOV₃