Experimental study on inhibitory effect of glycyrrhizin on HSV-1 in vitro

ZHAO Gaonian; et al

doi:10.13406/j.cnki.cyxb.2005.02.022

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Experimental study on inhibitory effect of glycyrrhizin on HSV-1 in vitro

更新时间：2024-01-17

- Experimental study on inhibitory effect of glycyrrhizin on HSV-1 in vitro
- Journal of Chongqing Medical University Issue 2, Pages: 243-245(2005)
- 作者机构：
  
  1. 重庆医科大学临床学院神经病学研究所
  2. 重庆医科大学临床学院神经病学研究所,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2005.02.022
  CLC： R285.5
- Published：2005，
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[1]赵高年,谢鹏,李平.甘草甜素对HSV-1抑制作用的实验研究[J].重庆医科大学学报,2005(02):243-245+268.

ZHAO Gaonian, et al. Experimental study on inhibitory effect of glycyrrhizin on HSV-1 in vitro. [J]. Journal of Chongqing Medical University (2):243-245(2005)
[1]赵高年,谢鹏,李平.甘草甜素对HSV-1抑制作用的实验研究[J].重庆医科大学学报,2005(02):243-245+268. DOI： 10.13406/j.cnki.cyxb.2005.02.022.

ZHAO Gaonian, et al. Experimental study on inhibitory effect of glycyrrhizin on HSV-1 in vitro. [J]. Journal of Chongqing Medical University (2):243-245(2005) DOI： 10.13406/j.cnki.cyxb.2005.02.022.

摘要

目的:观察甘草甜素对单纯疱疹病毒Ⅰ型的抑制作用

为临床更为有效的治疗单纯疱疹病毒性脑炎探索新的药物。方法:体外培养Vero细胞

分成甘草甜素在病毒吸附vero细胞后加入病毒、吸附前甘草甜素预处理细胞以及吸附前甘草甜素预处理病毒

通过观察空斑形成单位来评价甘草甜素对单纯疱疹病毒Ⅰ型的抑制作用

以及作用发生于病毒感染的哪个阶段。结果:病毒吸附vero细胞后加入甘草甜素能明显抑制病毒所致的空斑形成

IC50 为0 .5 6mmol/L。吸附前甘草甜素预处理细胞以及吸附前甘草甜素预处理病毒

对病毒所致的空斑形成无明显影响。结论:甘草甜素能明显地抑制单纯疱疹病毒Ⅰ型的复制

它的作用是发生在病毒穿入细胞后的阶段

不能阻止病毒对细胞的吸附

也不能直接灭活病毒

Abstract

Objective:To investigate the inhibitory effect of glycyrrhizin on HSV-1 in vitro

and to search a new drug against herpes simplex encephalitis.Methods:The Vero cells were cultured and divided into three groups:the group treated with glycyrrhizin after absorption of HSV-1 into the Vero cells

the group of pretreated cells and the group of pretreated HSV-1 with both glycyrrhizin before absorption of HSV-1 into the Vero cells.The plaque formation units were observed to evaluate the inhibitory effect of glycyrrhizin on HSV-1 and the stage when the virus replicating was inhibited.Results:Glycyrrhizin treatment after the absorption of virus into the cells obviously reduced the plaque formation units due to the virus infection and IC 50 was 0.56mM.Pretreated cells and pretreated HSV-1 with glycyrrhizin before absorption of HSV-1 into the cells did not reduce the plaque formation units.Conclusion:Glycyrrhizin can markedly inhibit the replication of HSV-1 in Vero cells.Glycyrrhizin can not inhibit the virus absorption and inactivate the virus.

关键词

甘草甜素单纯疱疹病毒Ⅰ型空斑形成单位

Keywords

GlycyrrhizinHSV- 1Plaque formation units

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