Serotonin stimulates the growth of bone marrow stromal cells

YI Qijian; et al

doi:10.13406/j.cnki.cyxb.2005.02.021

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Serotonin stimulates the growth of bone marrow stromal cells

更新时间：2024-01-17

- Serotonin stimulates the growth of bone marrow stromal cells
- Journal of Chongqing Medical University Issue 2, Pages: 239-242(2005)
- 作者机构：
  
  1. 重庆医科大学儿科学院心内科
  2. 香港中文大学儿科系
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2005.02.021
  CLC： R329.2
- Published：2005，
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[1]易岂建,杨默,庞雅轩,李桂霞,夏文杰,霍泰辉,宋银子.五羟色胺促进骨髓基质细胞生长初探[J].重庆医科大学学报,2005(02):239-242.

YI Qijian, et al. Serotonin stimulates the growth of bone marrow stromal cells. [J]. Journal of Chongqing Medical University (2):239-242(2005)
[1]易岂建,杨默,庞雅轩,李桂霞,夏文杰,霍泰辉,宋银子.五羟色胺促进骨髓基质细胞生长初探[J].重庆医科大学学报,2005(02):239-242. DOI： 10.13406/j.cnki.cyxb.2005.02.021.

YI Qijian, et al. Serotonin stimulates the growth of bone marrow stromal cells. [J]. Journal of Chongqing Medical University (2):239-242(2005) DOI： 10.13406/j.cnki.cyxb.2005.02.021.

摘要

目的:探讨五羟色胺（serotonin

5 -HT）对骨髓基质细胞（bonemarrowstromalcell

BMSC）体外生长的影响。方法:采用骨髓基质细胞集落培养技术（CFU -F）检测不同浓度5 -HT以及5 -HT分别与碱性成纤维细胞生长因子（basic-fibroblastgrowthfactor

bFGF）

血小板源性生长因子（ platelet-derivedgrowthfactor

PDGF）

血管内皮细胞生长因子（vascularendothelialgrowthfactor

VEGF）对小鼠及人BMSC体外培养的生长促进作用

反转录多聚酶链反应（RT -PCR）检测人BMSC表面5 -HT2型受体mRNA表达。结果:5 -HT不同浓度（ 5 0

10 0

2 0 0

5 0 0nM）对小鼠及人BMSC体外培养均有生长促进作用（P <0 .0 5 ）

显示最大作用浓度为2 0 0nM。5 -HT可增强bFGF

PDGF和VEGF对BMSC生长促进作用。人BMSC表面有5 -HT2A

2C受体mRNA表达。结论:5 -HT具有BMSC生长促进作用

并可增强bFGF

PDGF

VEGF促进BMSC的生长

其作用途径可能通过BMSC表面5 -HT2型受体

从而促进细胞生长

Abstract

Objective:To explore the effect of 5-hydroxytryptamine(5-HT) on proliferation of bone marrow stromal cells(BMSCs) in vitro.Methods:Murine BMSCs colony-forming unit assay and human bone marrow mononuclear cells count assay were used respectively to determine the effect on proliferation of BMSCs with different dose 5-HT(0

100

200

500nM) and 5-HT combined with basic fibroblast growth factor(bFGF)

platelet-derived growth factor(PDGF)

and ventricular endothelial growth factor(VEGF).Reversal transcript polymerase chain reaction(RT-PCR) was used to detect expression of mRNA of 5-HT2 receptor subtypes.Results:The results showed that 5-HT significantly stimulated murine colony-forming unit formation(CFU-F) proliferation in a dose-dependent manner(50 to 500nM) using murine bone marrow cells( n =3).Maxmum stimulation was obtained with 200nM of 5-HT( P =0.03).The effect of 5-HT on murine CFU-F proliferation was compared with that of bFGF

PDGF and VEGF at their optimal dose.The stimulating activity of 5-HT was the same as that of PDGF and VEGF

but lower than that of bFGF.A significantly enhancing effect was observed in 5-HT plus bFGF

PDGF

and VEGF

respectively.We also found that 5-HT

or 5-HT plus bFGF

PDGF

and VEGF had similar effect on human bone marrow stromal cells proliferation.There were expression of mRNA of 5-HT 2A

2B and 2C receptor subtypes on human bone marrow stromal cells.Conclusion:These data indicate that 5-HT has a significant mitogenic effect on BMSCs in vitro and enhance proliferating effect of bFGF

PDGF

and VEGF on BMSCs.This effect of 5-HT on BMSCs appears to be mediated via 5-HT2 receptor subtypes.

关键词

五羟色胺骨髓基质细胞受体

Keywords

5-HTbone marrow stromal cellReceptor

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