Effect of silencing mdrl gene by RNA interference with adriamycin on proliferation of SKOV<sub>3</sub>/ADM

WANG Pingling; et al

doi:10.13406/j.cnki.cyxb.2005.02.019

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Effect of silencing mdrl gene by RNA interference with adriamycin on proliferation of SKOV₃/ADM

更新时间：2024-01-17

- Effect of silencing mdrl gene by RNA interference with adriamycin on proliferation of SKOV₃/ADM
- Journal of Chongqing Medical University Issue 2, Pages: 232-235(2005)
- 作者机构：
  
  1. 重庆医科大学第二临床学院妇产科
  2. 重庆医科大学病毒性肝炎研究所,重庆,400010
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2005.02.019
  CLC： R737.31
- Published：2005，
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[1]王萍玲,胡丽娜,邓凯贤,张君,宋文鑫.RNA干扰沉默mdrl基因联合阿霉素对卵巢癌耐药细胞株SKOV_3/ADM增殖的抑制作用[J].重庆医科大学学报,2005(02):232-235.

WANG Pingling, et al. Effect of silencing mdrl gene by RNA interference with adriamycin on proliferation of SKOV₃/ADM. [J]. Journal of Chongqing Medical University (2):232-235(2005)
[1]王萍玲,胡丽娜,邓凯贤,张君,宋文鑫.RNA干扰沉默mdrl基因联合阿霉素对卵巢癌耐药细胞株SKOV_3/ADM增殖的抑制作用[J].重庆医科大学学报,2005(02):232-235. DOI： 10.13406/j.cnki.cyxb.2005.02.019.

WANG Pingling, et al. Effect of silencing mdrl gene by RNA interference with adriamycin on proliferation of SKOV₃/ADM. [J]. Journal of Chongqing Medical University (2):232-235(2005) DOI： 10.13406/j.cnki.cyxb.2005.02.019.

摘要

目的:观察RNA干扰沉默mdrl基因后化疗药物对卵巢癌耐药细胞株SKOV3 /ADM增殖的影响。方法:RT -PCR检测靶向于mdrl基因的小发夹状RNA（pshRNA -mdrl）对SKOV3 /ADM细胞内mdr1mRNA表达的影响;采用MTT法及软琼脂克隆形成实验测定pshRNA -mdrl联合化疗药物阿霉素对SKOV3 /ADM的增殖抑制作用。结果:pshRNA -mdrl能抑制SKOV3 /ADM细胞mdr1mRNA的表达

pshRNA -mdr1联合阿霉素明显抑制SKOV3 /ADM细胞的增殖。结论:靶向于mdr1的pshRNA联合阿霉素能显著抑制SKOV3 /ADM的增殖

增敏化疗

Abstract

Objective:To investigate the effect of RNA interference targeting mdrl gene with ADM on ovarian cancer resistant strain SKOV 3/ADM.Methods:The expression of mdrlmRNA after transfected pshRNA-mdrl was detected by RT-PCR;MTT test and clone forming test were applied to measure the inhibition combined with adriamycin on proliferation of SKOV 3/ADM strain.Results:These pshRNA-mdrls inhibited the expression of mdrlmRNA and proliferation of SKOV 3/ADM obviously when combined with adriamycin.Conclusion:The short hairpin RNA recombinant plasmid targeting mdrl gene combined with adriamycin inhibits the proliferation of SKOV 3/ADM and increases the sensitivity to chemotherapy.

关键词

卵巢癌耐药RNAimdr1基因

Keywords

ovarian cancerMultidrug resistance (MDR)RNAimdrl gene

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重庆医科大学病理生理教研室

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Effect of silencing mdrl gene by RNA interference with adriamycin on proliferation of SKOV3/ADM

DOI：10.13406/j.cnki.cyxb.2005.02.019

摘要

Abstract

关键词

Keywords

references

Effect of silencing mdrl gene by RNA interference with adriamycin on proliferation of SKOV₃/ADM