Study on experimental model of argon laser-induced choroidal neovascularization in the rabbits

QIAO Gang; et al

doi:10.13406/j.cnki.cyxb.2005.02.015

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Study on experimental model of argon laser-induced choroidal neovascularization in the rabbits

更新时间：2024-01-17

- Study on experimental model of argon laser-induced choroidal neovascularization in the rabbits
- Journal of Chongqing Medical University Issue 2, Pages: 216-219(2005)
- 作者机构：
  
  1. 重庆医科大学第二临床学院眼科
  2. 重庆医科大学第二临床学院眼科,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2005.02.015
  CLC： R-332
- Published：2005，
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[1]乔岗,周希瑗,张孝明,胡晓鹏,邓鑫,李琳.实验性兔脉络膜新生血管模型研究[J].重庆医科大学学报,2005(02):216-219.

QIAO Gang, et al. Study on experimental model of argon laser-induced choroidal neovascularization in the rabbits. [J]. Journal of Chongqing Medical University (2):216-219(2005)
[1]乔岗,周希瑗,张孝明,胡晓鹏,邓鑫,李琳.实验性兔脉络膜新生血管模型研究[J].重庆医科大学学报,2005(02):216-219. DOI： 10.13406/j.cnki.cyxb.2005.02.015.

QIAO Gang, et al. Study on experimental model of argon laser-induced choroidal neovascularization in the rabbits. [J]. Journal of Chongqing Medical University (2):216-219(2005) DOI： 10.13406/j.cnki.cyxb.2005.02.015.

摘要

目的:探讨氩激光创建有色家兔脉络膜新生血管（choroidalneovascularization

CNV）模型的可行性

初步探讨CNV的有关发生机制

为其进一步防治研究奠定基础。方法:健康有色家免2 0只（ 4 0只限）

每只兔一眼为实验眼

另眼为对照眼。以波长5 14 .5nm的氩绿激光（光斑直径5 0um

功率0 .7W

时间0 .1s）

在距视盘2～3个视盘直径的颢侧髓线上下视网膜处密集照射2 0个点。分别于光凝后3

2 1

30天进行眼底荧光造影（fundusfluoresceinangiography

FFA）及脉络膜造影（in docyaninegreenangiography

ICGA）。检查后处死动物（ 4只/次）

摘除眼球取眼后段组织制作石蜡切片。常规HE染色及用免疫组化（S -P法）检测血管内皮生长因子（VEGF）在视网膜的表达。结果:光凝后7天出现CNV

兔CNV发生率尚（ 6 3.8% ）

眼底造影和光镜下均有相应改变

FFA表现为视网膜血管无关的荧光素渗漏

ICGA见CNV充盈;光镜下可见到CNV结构;光凝后2周开始VEGF在视网膜神经节细胞以及内核层有表达。结论:氩激光诱导有色家免CNV模型成模时间短

成模率较高;VEGF参与了CNV的形成进程

Abstract

Objective:To evaluate the feasibility of argon laser-induced choroidal neovascularization(CNV)model in the pigmental rabbits in order to establish the foundation for studying the mechanism of CNV and for the development of treatment for CNV.Methods:Experimental eyes in 20 rabbits were received a series of 20 argon green laser(514.5nm) lesions per eye (700 mW

50um

0.1s).Fundus fluorescein angiography(FFA) and indocyanine green angiography (ICGA) were performed

then the rabbits were sacrificed immediately

the eyes were enucleated and processed for histopathologic and immunohistochemistry examination on days 3

30.Results:CNV first appeared on day 7 after photocoagulation

reaching the peak on day 21.The incidence of CNV was 63.0%.Disciform leakage staining appeared in the FFA.ICGA showed that the disciform area was filled with CNV.CNV was ascertained by light microscopy.The immunohistochemistry examination of VEGF was positive in ganglion cell layer and inner nuclear layer of retina from 2w after photocoagulation.Conclusion:The present studies demonstrated that argon laser photocoagulation could be used to produce choroidal neovascularization experimental model in the pigmental rabbits.CNV can be induced rapidly by the method

persists for a long period and at a high successful rate.VEGF plays a role in the formation of CNV.

关键词

脉络膜新生血管激光血管内皮生长因子

Keywords

choroidal neovascularizationlaserVEGF

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