The tffects of two tyrosine kinase inhibitors on the proliferation of colon cancer cell

ZHU Yujuan; et al

doi:10.13406/j.cnki.cyxb.2005.02.014

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The tffects of two tyrosine kinase inhibitors on the proliferation of colon cancer cell

更新时间：2024-01-17

- The tffects of two tyrosine kinase inhibitors on the proliferation of colon cancer cell
- Journal of Chongqing Medical University Issue 2, Pages: 213-215(2005)
- 作者机构：
  
  1. 三六三医院消化科
  2. 四川大学华西医院消化科,成都,610041
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2005.02.014
  CLC： R735.35
- Published：2005，
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[1]朱玉娟,王春晖.两种酪氨酸激酶抑制剂对结肠癌细胞增殖的影响[J].重庆医科大学学报,2005(02):213-215+265.

ZHU Yujuan, et al. The tffects of two tyrosine kinase inhibitors on the proliferation of colon cancer cell. [J]. Journal of Chongqing Medical University (2):213-215(2005)
[1]朱玉娟,王春晖.两种酪氨酸激酶抑制剂对结肠癌细胞增殖的影响[J].重庆医科大学学报,2005(02):213-215+265. DOI： 10.13406/j.cnki.cyxb.2005.02.014.

ZHU Yujuan, et al. The tffects of two tyrosine kinase inhibitors on the proliferation of colon cancer cell. [J]. Journal of Chongqing Medical University (2):213-215(2005) DOI： 10.13406/j.cnki.cyxb.2005.02.014.

摘要

目的:研究两种酪氨酸激酶抑制剂对体外培养的HT - 2 9结肠癌细胞生长的影响及可能机制。方法:用MTT比色法观察不同浓度的Genistein及TyrphostinAG14 78对体外培养的结肠癌细胞生长的影响;免疫细胞化学法检测两种酪氨酸激酶抑制剂（Tyrosinekinaseinhibitors

TKIs）对肿瘤细胞PCNA表达的影响;Westernblot技术分析Genistein及TyrphostinAG14 78对结肠癌细胞ERK - 1及pERK表达的影响。结果:不同浓度的Genistein及TyrphostinAG14 78对HT - 2 9结肠癌细胞的增殖均具有抑制作用

并具有良好的量-效关系。免疫细胞化学检测表明

Genistein及TyrphostinAG14 78明显下调HT- 2 9细胞PCNA表达。蛋白质印迹检测发现

两种酪氨酸激酶抑制剂不抑制ERK表达

但能够抑制ERK磷酸化。结论:酪氨酸激酶抑制剂Genistein及TyrphostinAG14 78能有效抑制HT - 2 9结肠癌细胞生长

其作用机制与抑制肿瘤细胞ERK表达及磷酸化有关

Abstract

Objective:To investigate the effects of Genistein and Tyrphostin AG1478 on the proliferation of colon cancer cell HT-29 and its possible mechanism.Methods:The effect of two tyrosine kinase inhibitors (TKIs) on HT-29 cell lines was studied by using MTT assay.Immunocytochemical staining was performed with PCNA polyclonal antibody in HT-29 cells.Western blot assay was used to detect the expression of ERK-1 and pERK.Results:Both Genistein and Tyrphostin AG1478 significantly inhibit the growth of HT-29 cells and showed concentration dependence.Two tyrosine kinase inhibitors suppressed PCNA expression.Western blot revealed that Genistein and Tyrphostin not inhibit ERK-1 expression but inhibit phosphorylation of ERK.Conclusion:Tyrosine kinase inhibitors Genistein and Tyrphostin AG1478 can inhibit the growth of HT-29 colon cancer cells

the anti-proliferation mechanism may be related to down-regulate the expression of pERK.

关键词

结肠癌细胞外信号调节激酶酪氨酸激酶抑制剂

Keywords

colon cancerExtralcellular-signal regulated kinasetyrosine kinase inhibitors

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