Investigation methods of diagnosis of Chlamydia trachomatis by Gap ligase chain reaction

DENG Chun; et al

doi:10.13406/j.cnki.cyxb.2005.02.006

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Investigation methods of diagnosis of Chlamydia trachomatis by Gap ligase chain reaction

更新时间：2024-01-17

- Investigation methods of diagnosis of Chlamydia trachomatis by Gap ligase chain reaction
- Journal of Chongqing Medical University Issue 2, Pages: 183-186(2005)
- 作者机构：
  
  1. 重庆医科大学儿科学院新生儿科
  2. 重庆医科大学儿科学院外科实验室
  3. 重庆医科大学儿科学院分子生物实验室
  4. 重庆医科大学儿科学院组织培养室,重庆,400014
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2005.02.006
  CLC： R446.61
- Published：2005，
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[1]邓春,吴仕孝,余加林,郭春宝,邓兵,刘官信.缺口-连接酶链反应-酶联免疫吸附(Gap-LCR-ELISA)检测沙眼衣原体的方法学研究[J].重庆医科大学学报,2005(02):183-186+190.

DENG Chun, et al. Investigation methods of diagnosis of Chlamydia trachomatis by Gap ligase chain reaction. [J]. Journal of Chongqing Medical University (2):183-186(2005)
[1]邓春,吴仕孝,余加林,郭春宝,邓兵,刘官信.缺口-连接酶链反应-酶联免疫吸附(Gap-LCR-ELISA)检测沙眼衣原体的方法学研究[J].重庆医科大学学报,2005(02):183-186+190. DOI： 10.13406/j.cnki.cyxb.2005.02.006.

DENG Chun, et al. Investigation methods of diagnosis of Chlamydia trachomatis by Gap ligase chain reaction. [J]. Journal of Chongqing Medical University (2):183-186(2005) DOI： 10.13406/j.cnki.cyxb.2005.02.006.

摘要

目的:探讨敏感性和特异性好的沙眼衣原体（Chlamydiatrachomatis

CT）感染的诊断方法。方法:肺炎患儿鼻咽拭子标本分别经细胞培养及质粒探针连接酶链反应分析;计算TC和G -LCR法敏感性、特异性、阳性预测值、阴性预测值

比较两种方法在检测CT感染中的差异。结果:按扩大金标准

真阳性共4 9例

阴性共344例。TC法假阴性12例

配对χ2 检验显示:G -LCR -ELISA与TC比较差异显著

G -LCR优于TC。结论:LCR方法是一种快速而准确的检测CT方法

LCR法敏感性高于TC

LCR法特异性高。

Abstract

Objective:To investigate the method with good sensitivity

specificity

reliability for diagnosis of Chlamydia trachomatis.Methods:Inoculate the nasopharyngeal swabs to detect the Chlamydia trachomatis(CT) with McCoy cell culture and plasmid gene probes labeled with biotin ligase-chain reaction.Then calculate the sensitivity

specificity

positive predicative value(PPV) and negative predicative value(NPV) of both TC and G-LCR respectively.Compare the difference of the two methods.Results:There were 49 positive specimens and 344 negative by enlarged gold standard.There was significant difference with cell culture

G-LCR-PAGE

G-LCR-ELISA by two-related-samples χ 2 tests( P <0.05).Conclusion:LCR assay is a kind of rapid and accurate method to diagnose Chlamydia trachomatis infection

compared with cell culture.LCR is simple

sensitive and suitable for clinical application.

关键词

沙眼衣原体连结酶链反应鼻咽拭子

Keywords

Chlamydia trachomatisLigase-chain reactionNasopharyngeal swab

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Department of Neonatology,the Children’s Hospital,Chongqing Medical University

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重庆医科大学儿科学院新生儿科

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