Study on biocompatability of nano-hydroxyapatite crystals and polyamide 66 composite with human embryonic fibroblast

MENG Chunyang; et al

doi:10.13406/j.cnki.cyxb.2005.02.003

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Study on biocompatability of nano-hydroxyapatite crystals and polyamide 66 composite with human embryonic fibroblast

更新时间：2024-01-17

- Study on biocompatability of nano-hydroxyapatite crystals and polyamide 66 composite with human embryonic fibroblast
- Journal of Chongqing Medical University Issue 2, Pages: 169-172(2005)
- 作者机构：
  
  1. 重庆医科大学临床学院骨科
  2. 重庆医科大学组胚教研室,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2005.02.003
  CLC： R318.08
- Published：2005，
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[1]孟纯阳,安洪,蒋电明,刘永刚.纳米羟基磷灰石/聚酰胺66与人胚胎成纤维细胞的生物相容性研究[J].重庆医科大学学报,2005(02):169-172.

MENG Chunyang, et al. Study on biocompatability of nano-hydroxyapatite crystals and polyamide 66 composite with human embryonic fibroblast. [J]. Journal of Chongqing Medical University (2):169-172(2005)
[1]孟纯阳,安洪,蒋电明,刘永刚.纳米羟基磷灰石/聚酰胺66与人胚胎成纤维细胞的生物相容性研究[J].重庆医科大学学报,2005(02):169-172. DOI： 10.13406/j.cnki.cyxb.2005.02.003.

MENG Chunyang, et al. Study on biocompatability of nano-hydroxyapatite crystals and polyamide 66 composite with human embryonic fibroblast. [J]. Journal of Chongqing Medical University (2):169-172(2005) DOI： 10.13406/j.cnki.cyxb.2005.02.003.

摘要

目的:研究新型骨修复重建复合材料纳米羟基磷灰石/聚酰胺6 6 （n -HA/PA6 6 ）对人胚胎成纤维细胞可能存在的细胞毒性

为该材料应用于临床提供实验依据。方法:采用人胚胎成纤维细胞

经材料及材料浸提液分别与细胞共培养等方式对n -HA/PA6 6进行细胞毒性测试

细胞形态观察法观察各组细胞在2 4h、4 8h、72h、5天各时相点的细胞形态学变化

细胞生长抑制法（MTT比色法）

测定各组1

7天细胞的相对增殖率。结果:材料表面的细胞黏附生长良好

各浸提液组和直接接触组分别与阴性对照组进行两两比较

各组对细胞的增殖均无显著影响（P >0 .0 5 ）

各浸提液组和直接接触组分别与阳性对照组进行两两比较显示有显著差异（P <0 .0 5 ）

材料的细胞毒性为0～1级。结论:体外试验结果显示

n -HA/PA6 6与人胚胎成纤维细胞相容性好

符合GB/T16 886 .5 - 1997-ISO 10 993- 5 :1992规定的医用材料相应标准[1] 。

Abstract

Objective:To evaluate the biocompatability of nano-hydroxyapatite crystals and polyamide composite(n-HA/PA66) with the human embryonic fibroblast(HEFs)

to provide an experimental evidence for its clinical application.Methods:The morphological observation was made and pictures of HEFs were taken 1d

and 7d after co-culture with extract of n-HA/PA66

and direct contact with n-HA/PA66.To determine absorbance of every hole under 500 nm with enzyme linked immunity instrument after 1d

and 7d of contact of n-HA/PA66 extract with HEF cells

and direct contact with n-HA/PA66.In the meanwhile calculate the relative multiplication rate of cells

and evaluate them by six degree tests for cytotoxicity.Results:The HEFs in direct contact culture with composite were attached and proliferated well on the materials surface.The growth and proliferation of cultured cells was not significantly inhibited between every composite group with the negative group ( P >0.05).There was significant difference between every composite group with the positive group( P <0.05).In this experiment the toxicity of n-HA/PA66 to HEF cells ranges from zero to one degree.Conclusion:n-HA/PA66 have good biocompatibility with human embryonic fibroblast(HEFs) in vitro

up to the standard of International Organization for Standardization(ISO).

关键词

纳米羟基磷灰石聚酰胺复合材料细胞相容性

Keywords

NanometerhydroxyapatitePolyamideCompositeCytocompatibility

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