Variation of virulence in galE knock-out mutant of Campylobacter jejuni

SHU Xiaomei; et al

doi:10.13406/j.cnki.cyxb.2005.02.002

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Variation of virulence in galE knock-out mutant of Campylobacter jejuni

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- Variation of virulence in galE knock-out mutant of Campylobacter jejuni
- Journal of Chongqing Medical University Issue 2, Pages: 166-168(2005)
- 作者机构：
  
  1. 重庆医科大学儿科学院儿科研究所
  2. 重庆医科大学儿科学院儿科研究所,重庆,400014
  3. 遵义医学院附院儿科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2005.02.002
  CLC： R378
- Published：2005，
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[1]束晓梅,蔡方成.galE基因敲除后空肠弯曲菌变异株毒力的改变[J].重庆医科大学学报,2005(02):166-168.

SHU Xiaomei, et al. Variation of virulence in galE knock-out mutant of Campylobacter jejuni. [J]. Journal of Chongqing Medical University (2):166-168(2005)
[1]束晓梅,蔡方成.galE基因敲除后空肠弯曲菌变异株毒力的改变[J].重庆医科大学学报,2005(02):166-168. DOI： 10.13406/j.cnki.cyxb.2005.02.002.

SHU Xiaomei, et al. Variation of virulence in galE knock-out mutant of Campylobacter jejuni. [J]. Journal of Chongqing Medical University (2):166-168(2005) DOI： 10.13406/j.cnki.cyxb.2005.02.002.

摘要

目的:研究galE基因敲除后空肠弯曲菌变异株的毒力改变。方法:使用Hep - 2细胞进行空肠弯曲菌野生株（CJHB9313）和galE变异株的黏附和侵袭实验

并比较两者血清敏感性和运动力的变化。结果:发现亲代株CJHB93及galE变异株对Hep - 2细胞黏附力分别为8.3%及1.2 %

变异株的黏附力仅为亲代株的14 .5 %

有显著差异（P <0 .0 1） ;CJHB9313及变异株对Hep - 2细胞侵袭力分别为5 .6 %及0 .3%

变异株侵袭力仅为亲代株的5 .4 %

差异也具显著性（P <0 .0 1） ;还发现亲代株及变异株对血清的敏感性也发生变化

与10 %血清孵育30min及6 0min后

前者存活77.6 %及6 7.0 %

后者存活33.6 %及8.4 %

均存在显著差异（P <0 .0 5 ） ;但变异株的运动力与亲代株无明显差异。结论:galE变异株的毒力明显减低

可能成为减毒CJ活菌苗的候选株

Abstract

Objective:To explore the change in virulence of Campylobacter jejuni (CJ) mutant after galE gene knock-out.Methods: Using Hep-2 cells

adherence and invasion assays were performed in the galE mutant and the parent strain

meanwhile

serum sensitivity assay and motility test were made in them. Results:The galE mutant showed a reduction in its ability to adhere to and invade Hep-2 cells. The ability of the galE mutant and parent strain to adhere to Hep-2 cells were 1.2 % and 8.3% respectively

and adherence ability of the former only was 14.5% of that of the latter. The difference was significant ( P <0.01). The ability of the galE mutant and parent strain to invade Hep-2 cells were 0.3% and 5.6% respectively

and invasion ability of the former only was 5.4% of that of the latter. The difference was also significant ( P <0.01).Furthermore

the sensitivity of both to sera were compared by incubated parental strain and galE mutant in 10% normal serum pools at 37℃. Bacterial counts were determined at 30 and 60 min after incubation. After 30mm

60 min of incubation

the survival of parental strain and galE mutant were 77.6%

67.0% and 33.6%

8.4% respectively. The survival of the galE mutant was significantly reduced compared to that of the parental strain ( P <0.05). The mobility test showed no difference between mutants and parental strains.Conclusion:These results indicate that the galE mutants with a significant reduction in virulence may have a future role as a live attenuated vaccine candidate.

关键词

空肠弯曲菌变异株毒力侵袭黏附

Keywords

Campylobacter jejuniMutantVirulenceinvasionAdhesion

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