IsoIation and 2-DE analysis of mitochondria of cultured hepatoma cells

LI Xing; et al

doi:10.13406/j.cnki.cyxb.2005.02.001

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IsoIation and 2-DE analysis of mitochondria of cultured hepatoma cells

更新时间：2024-01-17

- IsoIation and 2-DE analysis of mitochondria of cultured hepatoma cells
- Journal of Chongqing Medical University Issue 2, Pages: 163-165(2005)
- 作者机构：
  
  1. 重庆医科大学医学检验系
  2. 贵阳医学院医学检验系
  3. 第三军医大学野战外科研究所
  4. 重庆医科大学医学检验系,重庆,400016
  5. ,贵阳,550004
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2005.02.001
  CLC： R735.7
- Published：2005，
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[1]李兴,邱宗荫,潘卫,田昆仑,邱峰.肝癌细胞线粒体的纯化及双向凝胶电泳分析[J].重庆医科大学学报,2005(02):163-165+177.

LI Xing, et al. IsoIation and 2-DE analysis of mitochondria of cultured hepatoma cells. [J]. Journal of Chongqing Medical University (2):163-165(2005)
[1]李兴,邱宗荫,潘卫,田昆仑,邱峰.肝癌细胞线粒体的纯化及双向凝胶电泳分析[J].重庆医科大学学报,2005(02):163-165+177. DOI： 10.13406/j.cnki.cyxb.2005.02.001.

LI Xing, et al. IsoIation and 2-DE analysis of mitochondria of cultured hepatoma cells. [J]. Journal of Chongqing Medical University (2):163-165(2005) DOI： 10.13406/j.cnki.cyxb.2005.02.001.

摘要

目的:建立纯化肝癌细胞线粒体的方法

通过双向电泳及酶活力测定确认纯化效果。方法:使用蔗糖密度梯离心法分离纯化线粒体

双向凝胶电泳分析纯化过程中样品蛋白质图谱的变化。结果:通过标志酶活力的监测

梯度离心后线粒体纯提高近12倍

线粒体蛋白质在双向凝胶电泳中得到很好的显现。结论:使用蔗糖密度梯度心法可有效地分离纯化线粒体

为线粒体蛋白质组后续研究奠定了基础

Abstract

Objective:To make the isolation and 2-DE analysis of mitochondria

in order to prepare the following proteomic study on mitochondria of hepatoma-cell. Methods:Mitochondria was separated from cell homogenate by means of density gradient centrifugation

and 2-DE was conducted to examine the protein profile of mitochondrial preparations.Results:The increase in purity of mitochondria was found to be 12 times by density gradient centrifugation.Mitochondrial proteins were displayed well in the 2-DE pattern after purification.Conclusion:The isolation procedure is practicable

which provides a basis for the following proteomic study on mitochondria.

关键词

线粒体梯度离心双向凝胶电泳

Keywords

mitochondriaDensity gradient centrifugationtwo-dimensional gel electrophoresis

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