Preparation of immunoliposomes encapsulating alkaline phosphatase

HUANG Jinjiang; et al

doi:10.13406/j.cnki.cyxb.2005.01.026

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Preparation of immunoliposomes encapsulating alkaline phosphatase

更新时间：2024-01-17

- Preparation of immunoliposomes encapsulating alkaline phosphatase
- Journal of Chongqing Medical University Issue 1, Pages: 92-94(2005)
- 作者机构：
  
  1. 重庆医科大学医学检验系
  2. 四川省人民医院检验科
  3. 重庆医科大学医学检验系,重庆,400016
  4. ,成都,610072
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2005.01.026
  CLC： R392.33
- Published：2005，
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[1]黄锦江,黄文方,刘小琦,杨明清,涂植光.包裹碱性磷酸酶免疫脂质体的制备[J].重庆医科大学学报,2005(01):92-94.

HUANG Jinjiang, et al. Preparation of immunoliposomes encapsulating alkaline phosphatase. [J]. Journal of Chongqing Medical University (1):92-94(2005)
[1]黄锦江,黄文方,刘小琦,杨明清,涂植光.包裹碱性磷酸酶免疫脂质体的制备[J].重庆医科大学学报,2005(01):92-94. DOI： 10.13406/j.cnki.cyxb.2005.01.026.

HUANG Jinjiang, et al. Preparation of immunoliposomes encapsulating alkaline phosphatase. [J]. Journal of Chongqing Medical University (1):92-94(2005) DOI： 10.13406/j.cnki.cyxb.2005.01.026.

摘要

目的制备包裹碱性磷酸酶的免疫脂质体并评价实验方法。方法采用逆相蒸发法、薄膜分散法制备包裹碱性磷酸酶的脂质体

应用戊二醛交联法、抗体衍生化法将脂质体与兔抗人IgG结合

使脂质体既包裹酶又具有免疫活性。对制备方法进行了分析比较。结果应用逆相蒸发法制备的脂质体数量多

圆整均匀

具有较高的碱性磷酸酶活性;以戊二醛为交联剂结合抗体方便、高效、实用。结论通过选择适当的实验方法

制备了具有酶和抗体活性的免疫脂质体。

Abstract

Objective:To prepare immunoliposomes encapsulating alkaline phosphatase and to evaluate the methods.Methods:The liposomes containing alkaline phosphatase were prepared by reverse phase evaporation or film dispersion.Liposomes and rabbit anti-human IgG were conjugated by glutaraldhyde or deriving antibody.Not only did the liposomes encapsulate alkaline phosphatase but also they had immune activity.All methods were analyzed and compared.Results:Many liposomes prepared by reverse phase evaporation had perfect shape and high bioactivity of alkaline phosphatase.The glutaraldhyde method conjugating antibody was convenient

efficient and practical.Conclusion:Immunoliposomes with bioactivity of enzyme and antibody are prepared through reasonable methods.

关键词

碱性磷酸酶免疫脂质体抗体制备

Keywords

alkaline phosphataseImmunoliposomeantibodyPreparation

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