The effects of recombinant human hepatocyte growth factor on proliferation and migration of human umbilical vein endothelial cells

TANG Bo; et al

doi:10.13406/j.cnki.cyxb.2005.01.024

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The effects of recombinant human hepatocyte growth factor on proliferation and migration of human umbilical vein endothelial cells

更新时间：2024-01-17

- The effects of recombinant human hepatocyte growth factor on proliferation and migration of human umbilical vein endothelial cells
- Journal of Chongqing Medical University Issue 1, Pages: 85-88(2005)
- 作者机构：
  
  1. 重庆医科大学第二临床学院血管外科
  2. 重庆大学生物工程学院
  3. 重庆医科大学第二临床学院血管外科,重庆,400010
  4. ,重庆,400044
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2005.01.024
  CLC： R329.2
- Published：2005，
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[1]唐博,罗文军,严润彬,陈若飞.肝细胞生长因子促血管内皮细胞增殖及迁移的研究[J].重庆医科大学学报,2005(01):85-88.

TANG Bo, et al. The effects of recombinant human hepatocyte growth factor on proliferation and migration of human umbilical vein endothelial cells. [J]. Journal of Chongqing Medical University (1):85-88(2005)
[1]唐博,罗文军,严润彬,陈若飞.肝细胞生长因子促血管内皮细胞增殖及迁移的研究[J].重庆医科大学学报,2005(01):85-88. DOI： 10.13406/j.cnki.cyxb.2005.01.024.

TANG Bo, et al. The effects of recombinant human hepatocyte growth factor on proliferation and migration of human umbilical vein endothelial cells. [J]. Journal of Chongqing Medical University (1):85-88(2005) DOI： 10.13406/j.cnki.cyxb.2005.01.024.

摘要

目的探讨重组人肝细胞生长因子（rhHGF）对人脐静脉血管内皮细胞（HUVEC）增殖和迁移的影响及其促血管生成作用。方法从脐静脉体外分离培养HUVEC

采用四甲基偶氮唑蓝法（MTT）观察rhHGF对HUVEC的增殖作用

倒置显微镜观察HUVEC的迁移

透射电镜观察超微结构的改变及流式细胞仪检测细胞周期的改变。结果（1）rhHGF对培养的HUVEC有显著的促增殖和迁移作用

且成浓度和时间依赖性。（2）rhHGF促使HUVEC细胞超微结构的改变

线粒体和粗面内质网增多

常染色质增多（3）细胞周期分布改变S期、G2/M期细胞增多。结论rhHGF能显著促进HUVEC的增殖和迁移

提示其可能具有促血管生成作用。

Abstract

Objective:To investigate the effects of hepatocyte growth factor on proliferation and migration of human umbilical vein endothelial cells

and to further study the angiogenesis of HGF.Methods:The cultured endothelial cells in vitro were isolated from human umbilical vein.The proliferation of HUVECs were measered by MTT and their migration were observed using reverse microscope

The changes of celluar ultrastructure were observed under electron microscope and flow cytometry was applied to observe the effects of rhHGF on cell cycle.Results:rhHGF could promote the proliferation and migration of HUVECs dose-dependently and time-dependently.There were obvious changes in celluar structure

rhHGF could increase mitochondrion

rough endoplasmic reticulum and euchromatin.Conclusion:rhHGF could promote the proliferation and migration of HUVECs significantly

which suggests that HGF might have the effect of angiogenesis.

关键词

肝细胞生长因子血管内皮细胞增殖迁移细胞周期

Keywords

hepatocyte growth factorEndothelial cellsproliferationmigrationcell cycle

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