Influence of specific cyclooxygenase-2 inhibitor on TNF-α,IL-6 and expression CD14 positive cells in lupus MRL/lpr mice

ZHONG Qing; et al

doi:10.13406/j.cnki.cyxb.2005.01.020

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Influence of specific cyclooxygenase-2 inhibitor on TNF-α,IL-6 and expression CD14 positive cells in lupus MRL/lpr mice

更新时间：2024-01-17

- Influence of specific cyclooxygenase-2 inhibitor on TNF-α,IL-6 and expression CD14 positive cells in lupus MRL/lpr mice
- Journal of Chongqing Medical University Issue 1, Pages: 71-74(2005)
- 作者机构：
  
  1. 重庆医科大学临床学院肾内科
  2. 重庆医科大学临床学院肾内科,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2005.01.020
  CLC： R593.241
- Published：2005，
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[1]钟清,甘华,肖刚.特异性COX-2抑制剂对小鼠狼疮性肾炎CD14~+表达及TNF-α、IL-6的影响[J].重庆医科大学学报,2005(01):71-74.

ZHONG Qing, et al. Influence of specific cyclooxygenase-2 inhibitor on TNF-α,IL-6 and expression CD14 positive cells in lupus MRL/lpr mice. [J]. Journal of Chongqing Medical University (1):71-74(2005)
[1]钟清,甘华,肖刚.特异性COX-2抑制剂对小鼠狼疮性肾炎CD14~+表达及TNF-α、IL-6的影响[J].重庆医科大学学报,2005(01):71-74. DOI： 10.13406/j.cnki.cyxb.2005.01.020.

ZHONG Qing, et al. Influence of specific cyclooxygenase-2 inhibitor on TNF-α,IL-6 and expression CD14 positive cells in lupus MRL/lpr mice. [J]. Journal of Chongqing Medical University (1):71-74(2005) DOI： 10.13406/j.cnki.cyxb.2005.01.020.

摘要

目的探讨特异性COX-2抑制剂对MRL/lpr自发狼疮小鼠的CD14+表达及TNF-α、IL-6的影响。方法用特异性环氧化酶-2（COX-2）抑制剂（Rofecoxib10·kg-1·d-1）治疗12周龄雌性MRL/lpr自发狼疮小鼠

并与未干预组对照。12周后用放射免疫方法检测小鼠血清和尿中肿瘤坏死因子（Tumornecrosisfactor-α

TNF-α）、白细胞介素-6（Interleukin-6

IL-6）及尿血栓索B2（TXB2）的变化;并观察小鼠尿蛋白、血肌酐、肾组织病理改变及CD14+细胞数的变化。结果治疗12周后

治疗组与对照组比较

小鼠血清和尿中TNF-α、IL-6水平降低（P<0.05）;24h尿蛋白排泄量、血肌酐水平及肾组织CD14+细胞数差异均有显著性意义（P<0.05）;肾小球基底膜（GBM）增厚程度差异有显著性意义（P<0.05）。结论特异性COX-2抑制剂治疗MRL/lpr自发狼疮小鼠

可能通过抑制单核/巨噬细胞在肾组织中的浸润

降低血清和尿中TNF-α、IL-6水平

从而减轻了MRL/lpr小鼠肾损害

对LN有较好的治疗作用。

Abstract

Objective:To explore the underlying molecular mechanisms of specific cyclooxygenase-2 inhibitor(Rofecoxib)and to evaluate its efficacy on MRL/lpr mice.Methods:12-week female MRL/lpr mice were given Rofecoxib (10mg·kg -1 ·d -1 )for 12 weeks.Suppressive effect of the drugs on serum and urinary levels of tumor necrosis factor-α(TNF-α)

interleukin-6(IL-6) and urinary TXB 2 level were determined by using radio-immunological assay.Changes in proteinuria

serum creatinine

pathology of ltidney and the expression of CD14 in renal tissues were observed.Results:Compared with the ones in the control group

mice treated with Rofecoxib had lower levels of proteinuria( P <0.01)

serum creatinine( P <0.01)

urinary TXB 2 ( P <0.01)

TNF-α and IL-6( P <0.05) and the thickness of glomerular base membrane (GBM) ( P <0.05).The number of CD14 expression of the positive cells in renal tissue significantly decreased.Conclusion:TNF-α and IL-6 may take part in immune pathogenesis of lupus nephritis(LN).Serum and urinary levels of TNF-α

IL-6 may be useful indicators for monitoring disease degree of renal damage in LN.Rofecoxib may have a significant effect on inhibiting the production of INF-α

IL-6 in the monocytes/macrophages cells.Rofecoxib may decrease the levels of serum and urinary TNF-α

IL-6 and ameliorate renal injuries in lupus MRL/lpr mice.The specific cyclooxygenase-2 inhibitor has a renoprotective effect on MRL-lpr/lpr mice.

关键词

特异性COX-2抑制剂TNF-αIL-6狼疮性肾炎

Keywords

Specific cyclooxygenase-2 inhibitorTumor necrosis factor-αInterleukin-6lupus nephritis

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