Effects of oxymartine on expression of P-glycoprotein in HNE-1 cell line radiation therapy

WANG Chi; et al

doi:10.13406/j.cnki.cyxb.2005.01.005

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Effects of oxymartine on expression of P-glycoprotein in HNE-1 cell line radiation therapy

更新时间：2024-01-17

- Effects of oxymartine on expression of P-glycoprotein in HNE-1 cell line radiation therapy
- Journal of Chongqing Medical University Issue 1, Pages: 16-19(2005)
- 作者机构：
  
  1. 重庆医科大学基础医学院化学教研室
  2. 重庆医科大学临床学院耳鼻喉科
  3. 重庆医科大学药学系,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2005.01.005
  CLC： R739.63
- Published：2005，
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[1]王驰,陈鸿雁,舒艳,邱宗荫.氧化苦参碱对放疗鼻咽癌HNE-1细胞P-糖蛋白表达影响[J].重庆医科大学学报,2005(01):16-19.

WANG Chi, et al. Effects of oxymartine on expression of P-glycoprotein in HNE-1 cell line radiation therapy. [J]. Journal of Chongqing Medical University (1):16-19(2005)
[1]王驰,陈鸿雁,舒艳,邱宗荫.氧化苦参碱对放疗鼻咽癌HNE-1细胞P-糖蛋白表达影响[J].重庆医科大学学报,2005(01):16-19. DOI： 10.13406/j.cnki.cyxb.2005.01.005.

WANG Chi, et al. Effects of oxymartine on expression of P-glycoprotein in HNE-1 cell line radiation therapy. [J]. Journal of Chongqing Medical University (1):16-19(2005) DOI： 10.13406/j.cnki.cyxb.2005.01.005.

摘要

目的（1）氧化苦参碱对放射前后鼻咽癌HNE-1细胞有无生长抑制作用;（2）研究天然产物氧化苦参碱对鼻咽癌HNE-1细胞株放射前、后P-糖蛋白表达的影响。方法（1）MTT法检测长春新碱、维拉帕米、氧化苦参碱对放射前HNE-1细胞、放射后HNE-1（200）细胞生长抑制情况;（2）免疫化学法检测氧化苦参碱、维拉帕米对HNE-1（200）细胞P-糖蛋白表达的影响。结果（1）长春新碱、维拉帕米、氧化苦参碱对放射前、后细胞生长均有抑制作用

呈浓度依赖关系

随着浓度的增加抑制作用也增加;（2）高浓度VCR对放射治疗后细胞HNE-1（200）的抑制率比对HNE-1细胞的抑制率降低了近50%

P值小于0.01

有显著差异;氧化苦参碱、维拉帕米对两种实验细胞抑制没有显著差异。（3）免疫组化检测细胞P-糖蛋白表达

结果为HNE-1阴性

HNE-1（200）阳性

维拉帕米、氧化苦参碱分别作用于HNE-1（200）细胞后P-gp表达弱阳性。结论（1）氧化苦参碱对放射前后鼻咽癌HNE-1细胞有明显生长抑制作用;（2）放射后细胞HNE-1（200）对长春新碱产生耐药;（3）低毒性浓度氧化苦参碱具有与维拉帕米相似的作用

能使放射照射后鼻咽癌HNE-1细胞株P-糖蛋白的表达下降。

Abstract

Objective:To investigate the effects of oxymartine on cell growth and the changes of P-glycoprotein in HNE-1 cell line after exposed to fractionated X-irradiation.Methods:By MTT

the cell proliferation was observed both before X-irradiation and after X-irradiation conditions with the extent of vincristine

oxymartine and verapamil respectively.P-glycoprotein expression was tested by immunohistochemistory method SP.Results:(1)Vincristine

oxymartine and verapamil were capable of inhibiting cell growth on HNE-1 cell line and fractionated X-irradiated HNE-1(200)cell subline;(2)Vincristine was less inhibitory on cell growth in HNE-1(200)cell subline than HNE-1 cell line

and the difference had statistic significance;(3)P-glycoprotein expression in HNE-1 cell line was negative while HNE-1(200)cell subline positive;oxymartine with low inhibitory concentration could increase the expression of P-glycoprotein in HNE-1(200) cell subline

which is similar to that of verapamil.Conclusion:(1)Oxymartine is capable of inhibiting cell growth on HNE-1 cell line and fractionated X-irradiated HNE-1(200)cell subline;(2)HNE-I(200)cell subline is resistant to vincristine;(3)Oxymartine with low inhibitory concentration could increase the expression of P-glycoprotein in HNE-1(200)cell subline.which is similar to that of verapamil.

关键词

鼻咽癌HNE-1细胞株P-糖蛋白MTT法氧化苦参碱

Keywords

HNE-1 cell lineP-glycoproteinMTTOxymartine

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