The effects of all-trans retinoic acid on proliferation and differentiation in ovarian cancer cell line COC1

YANG Min; et al

doi:10.13406/j.cnki.cyxb.2005.01.004

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The effects of all-trans retinoic acid on proliferation and differentiation in ovarian cancer cell line COC1

更新时间：2024-01-17

- The effects of all-trans retinoic acid on proliferation and differentiation in ovarian cancer cell line COC1
- Journal of Chongqing Medical University Issue 1, Pages: 12-15(2005)
- 作者机构：
  
  1. 重庆医科大学基础医学院病理学教研室
  2. 重庆医科大学基础医学院病理学教研室,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2005.01.004
  CLC： R737.31
- Published：2005，
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[1]杨敏,赵涌,林晓.全反式维甲酸对人卵巢癌细胞株COC1增殖分化的影响[J].重庆医科大学学报,2005(01):12-15.

YANG Min, et al. The effects of all-trans retinoic acid on proliferation and differentiation in ovarian cancer cell line COC1. [J]. Journal of Chongqing Medical University (1):12-15(2005)
[1]杨敏,赵涌,林晓.全反式维甲酸对人卵巢癌细胞株COC1增殖分化的影响[J].重庆医科大学学报,2005(01):12-15. DOI： 10.13406/j.cnki.cyxb.2005.01.004.

YANG Min, et al. The effects of all-trans retinoic acid on proliferation and differentiation in ovarian cancer cell line COC1. [J]. Journal of Chongqing Medical University (1):12-15(2005) DOI： 10.13406/j.cnki.cyxb.2005.01.004.

摘要

目的探讨全反式维甲酸（ATRA）对人卵巢癌细胞株COC1增殖分化的影响。方法选用人卵巢癌细胞株COC1进行体外培养

以不同浓度的ATRA处理COC1细胞

MTT比色法检测细胞增殖抑制情况;流式细胞仪进行细胞周期时相分析;光镜及透射电镜进行形态学观察;免疫细胞化学法测定细胞内增殖细胞核抗原Ki-67的表达;酶联免疫法测定培养液中卵巢癌标志物CA125水平的变化。结果ATRA浓度为10-7～10-5mol/L时

均明显抑制COC1细胞的增殖;ATRA处理COC1细胞后G0/G1期细胞增加

S期细胞减少。经ATRA处理的COC1细胞发生显著形态学分化

Ki-67表达减弱

培养液中CA125的表达水平降低。结论ATRA能明显抑制人卵巢癌细胞株COC1的生长增殖并诱导部分细胞向良性分化。

Abstract

Objective:To investigate the effects of all-trans retinoic acid (ATRA) on prolifetation and differentiation in ovarian cancer cell line COC1 in vitro.Methods:Human ovarian cancer cell line COC1 was cultured in medium containing ATRA in different concentrations in vitro.Cell proliferation inhibition was tested by MTT assay.The cell cycle was analyzed by flow cytometry(FCM).Morphological changes of cell differentiation were observed by light and transmission electron microscopy

and the expression of Ki-67 in cancer cells were measured with SP immunohistochemistry.In addition

CA 125 expression in the culture medium was measured by ELISA.Results:ATRA of 10 -7 ～10 -5 mol/L inhibited COC1 cell proliferation significantly in a dose-dependent and time-dependent manner.With the increase of ATRA concentrations

the number of cells in G 0/G 1 phase were elevated

the number of cells in S phase were obviously decreased.COC1 cells after treated by ATRA showed striking morphological characteristics of differentiation.The expression of Ki-67 was decreased in COC1 after ATRA treatment.ATRA could also suppress the expression of CA125 tumor marker in COC1 cells.Conclusion:ATRA can both inhibit proliferation and induce differentiation of human ovarian cancer cell line COC1.These results may provide an effective and novel approach for clinical treatment of human ovarian cancer.

关键词

卵巢肿瘤维甲酸增殖分化

Keywords

Ovarian neoplasmsRetinoic acidproliferationdifferentiation

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