Effects of mouse blastocysts on invasive,adhesive and mobile capacity of human endometrial carcinoma cells in vitro

WANG Huanying; et al

doi:10.13406/j.cnki.cyxb.2005.01.003

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Effects of mouse blastocysts on invasive,adhesive and mobile capacity of human endometrial carcinoma cells in vitro

更新时间：2024-01-17

- Effects of mouse blastocysts on invasive,adhesive and mobile capacity of human endometrial carcinoma cells in vitro
- Journal of Chongqing Medical University Issue 1, Pages: 9-11(2005)
- 作者机构：
  
  1. 重庆医科大学生物医学工程系
  2. 重庆医科大学临床学院妇产科
  3. 重庆医科大学生物医学工程系,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2005.01.003
  CLC： R737.33
- Published：2005，
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[1]王焕英,赵,王媛,胡凯,姚珍薇,王智彪.与小鼠囊胚共培养后人子宫内膜癌细胞侵袭、粘附和运动性的变化[J].重庆医科大学学报,2005(01):9-11+19.

WANG Huanying, et al. Effects of mouse blastocysts on invasive,adhesive and mobile capacity of human endometrial carcinoma cells in vitro. [J]. Journal of Chongqing Medical University (1):9-11(2005)
[1]王焕英,赵,王媛,胡凯,姚珍薇,王智彪.与小鼠囊胚共培养后人子宫内膜癌细胞侵袭、粘附和运动性的变化[J].重庆医科大学学报,2005(01):9-11+19. DOI： 10.13406/j.cnki.cyxb.2005.01.003.

WANG Huanying, et al. Effects of mouse blastocysts on invasive,adhesive and mobile capacity of human endometrial carcinoma cells in vitro. [J]. Journal of Chongqing Medical University (1):9-11(2005) DOI： 10.13406/j.cnki.cyxb.2005.01.003.

摘要

目的了解小鼠囊胚体外对人子宫内膜癌细胞RL95-2侵袭力及粘附、运动性的影响作用。方法建立小鼠囊胚与人子宫内膜癌细胞RL95-2共培养模型

分别采用Matrigel人工重建基底膜侵袭实验、MTT法粘附实验和Transwell小室趋化性运动模型

来研究与小鼠囊胚共培养后

人子宫内膜癌细胞RL95-2体外侵袭能力、粘附性和运动性的改变。结果小鼠囊胚与人子宫内膜癌细胞RL95-2共培养后

RL95-2细胞穿过Matrigel人工基底膜的细胞数明显减少（14±5.66vs38.08±13.43

P<0.01）

30min和60min的粘附率均降低（P<0.01）

穿过Transwell小室聚碳酸脂微孔的细胞数也显著减少（24.69±1.57vs59.19±2.39

P<0.01）。结论小鼠囊胚可降低恶性肿瘤细胞的侵袭能力

及侵袭相关的粘附性和趋化运动性。

Abstract

Objective:This study was conducted to investigate the effects of mouse blastocysts on invasive

adhesive and mobile capacity of human endometrial carcinoma cells RL95-2 in vitro.Methods:RL95-2 cells were co-cultured with mouse blastocysts.Changes of invasive

mobile and adhesive capacity of RL95-2 cells were measured by the number of cells to penetrate polycarbonates coated with or without Matrigel

and adhesive rate by MTT after co-culture

respectively.Results:After co-culture with mouse blastocysts

number of RL95-2 cells to penetrate polycarbonates coated with or without Matrigel was significantly lower (14±5.66 vs 38.08±13.43 and 24.69±1.57 vs 59.19±2.39

respectively

P <0.01).Adhesive rate of RL95-2 cells was also significantly decreased at 30min and 60min ( P <0.01).Conclusion:Mouse blastocysts can make invasiveness of malignant tumor cells decrease

and change invasion-related adhesive and mobile capacity in vitro.

关键词

胚胎肿瘤细胞侵袭性共培养

Keywords

embryoTumor cellsInvasionco-culture

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Department of Infections Disease,The First Affiliated Hospital of Chongqing Medical University,Key Laboratory of Infectious and Parasitic Diseases

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Department of Obstetrics and Gynecology,the Second College of Clinical Medicine,Chongqing Medical University

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重庆医科大学实验动物中心

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