Experimental study on isolated human testes with ischemia-reperfusion injury

LI Ming; et al

doi:10.13406/j.cnki.cyxb.2005.01.001

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Experimental study on isolated human testes with ischemia-reperfusion injury

更新时间：2024-01-17

- Experimental study on isolated human testes with ischemia-reperfusion injury
- Journal of Chongqing Medical University Issue 1, Pages: 1-4(2005)
- 作者机构：
  
  1. 重庆医科大学临床学院普外科
  2. 四川大学基础医学与法医学院免疫学教研室
  3. 四川大学华西医院移植工程和移植免疫实验室,重庆,400016
  4. ,成都,610041
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2005.01.001
  CLC： R699
- Published：2005，
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[1]李明,蒋红梅,李幼平.人离体睾丸缺血再灌注损伤的实验性研究[J].重庆医科大学学报,2005(01):1-4.

LI Ming, et al. Experimental study on isolated human testes with ischemia-reperfusion injury. [J]. Journal of Chongqing Medical University (1):1-4(2005)
[1]李明,蒋红梅,李幼平.人离体睾丸缺血再灌注损伤的实验性研究[J].重庆医科大学学报,2005(01):1-4. DOI： 10.13406/j.cnki.cyxb.2005.01.001.

LI Ming, et al. Experimental study on isolated human testes with ischemia-reperfusion injury. [J]. Journal of Chongqing Medical University (1):1-4(2005) DOI： 10.13406/j.cnki.cyxb.2005.01.001.

摘要

目的探讨人离体睾丸的缺血再灌注损伤变化规律

为临床行睾丸移植提供理论依据。方法采用13人捐赠尸睾

用250ml0℃～4℃高渗枸橼酸盐嘌呤液（HCA液）灌洗后冷存

再以500ml37℃该液再灌注

不同时间点取材作组织学及酶组织化学检查。结果冷缺血18小时开始出现血管内皮细胞肿胀变圆、空泡样变

24小时内皮细胞变性脱落

睾丸曲细精管基膜与生精上皮剥离

生精细胞变性脱落

间质水肿等

病变随冷缺血时间延长而加重;酶组化结果显示单纯冷保存18小时后睾丸组织乳酸脱氢酶（LDH）活性升高

琥珀酸脱氢酶（SDH）活性24小时后升高。经37℃复温再灌注后损伤明显加重。结论人睾丸对缺血再灌注损伤较敏感

冷保存18小时内仅有血管内皮细胞轻微损伤

睾丸组织LDH和SDH活性分别在冷保存1小时和24小时后升高

以上变化经37℃复温再灌注后明显加重。

Abstract

Objective:To investigate the injury on isolated cadaver testes induced by ischemia-reperfusion (I/R).Methods:13 isolated cadaver testes contributed by 13 persons were preserved under 0℃～4℃ hypothermia and then reperfused under 37℃.Histology and histochemical changes were observed.Results:4℃ cold ischemia in 18 hours only could induce trivial swelling and vascular degeneration of endothelial cells(ECs).Obvious pathologic changes occurred after 24 hours

including detachment of ECs

separation between basement membrane and seminiferous epitelium

malalignment of spermatogenous cell and edema of mesenchyme.Injury was worse along with the prolongation of cold preservation time.Changes of LDH and SDH activities were found by histochemical staining.Reperfusion following 6 hours ischemia induced tissue injury and unusual enzyme activity.All changes were more obvious after reperfusion following 12

24 or 36 hours of cold ischemia.Conclusion:HCA can preserve testes for 18 hours.Testes can’t keep normal histological and histochemical structure beyond 24 hours of preservation under 4℃ hypothermia

and 37℃ reperfusion can make injury worse.

关键词

睾丸缺血再灌注损伤移植酶组织化学

Keywords

Testesischemia-reperfusion injuryTransplantEnzyme histochemical staining

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