Fast assay of vitamin C in body fluids by iodometry with UV absorbance detection

ZHU Xiaoyun; et al

doi:10.13406/j.cnki.cyxb.2004.06.044

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Fast assay of vitamin C in body fluids by iodometry with UV absorbance detection

更新时间：2024-01-17

- Fast assay of vitamin C in body fluids by iodometry with UV absorbance detection
- Journal of Chongqing Medical University Issue 6, Pages: 841-842(2004)
- 作者机构：
  
  1. 重庆医科大学生物化学教研室
  2. 重庆医科大学2001级
  3. 重庆医科大学生物化学教研室,重庆,400016
  4. 七年本科学生,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2004.06.044
  CLC： R96
- Published：2004，
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[1]朱小云,李生兵,郭睿,廖飞,王咏梅,左渝萍.紫外吸收碘量法快速测定体液维生素C[J].重庆医科大学学报,2004(06):841-842+848.

ZHU Xiaoyun, et al. Fast assay of vitamin C in body fluids by iodometry with UV absorbance detection. [J]. Journal of Chongqing Medical University (6):841-842(2004)
[1]朱小云,李生兵,郭睿,廖飞,王咏梅,左渝萍.紫外吸收碘量法快速测定体液维生素C[J].重庆医科大学学报,2004(06):841-842+848. DOI： 10.13406/j.cnki.cyxb.2004.06.044.

ZHU Xiaoyun, et al. Fast assay of vitamin C in body fluids by iodometry with UV absorbance detection. [J]. Journal of Chongqing Medical University (6):841-842(2004) DOI： 10.13406/j.cnki.cyxb.2004.06.044.

摘要

目的 :考察用紫外吸收碘量法快速测定体液维生素C的可行性。方法 :用碘乙酸封闭巯基

三氯乙酸沉淀蛋白质

二氯甲烷抽提除去干扰色素。测定处理后体液样品与碘反应 30s前后 35 3nm吸收变化。结果 :测定反应前或后的光吸收变异<5 %

反应前后光吸收变化同体系VC含量成正比

响应曲线斜率与三碘复合物的消光系数接近

样品中VC回收率 >90 %

可测定反应体系 5～ 30 μmol/L的VC。结论 :本方法有望用于体液VC的快速测定。

Abstract

Objective:To investigate the feasibility of fast assay of vitamin C (VC) in body fluids by iodometry with UV absorbance detection.Methods:The sample was pretreated with iodoacetic acid to block sulfhydryl group.Protein was precipitated by trichloroacetic acid

and chromogen was extracted with chloromethylene.The change of absorbance after 30 s reaction of the pretreated sample with iodine was measured.Results:The assay of absorbance before and after 30 s reaction of the pretreated sample with iodine showed the variation coefficient within 5%

and the recovery of VC was above 90%.There was linear response of the absorbance change to the amount of VC from body fluids with slope consistent to that for iodine.Conclusion:This method may be desirable for fast assay of VC in body fluids.

关键词

维生素C碘量法紫外吸收检测

Keywords

vitamin CIodometryUV absorbance detection

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