Analysis of drug resistance and genotype of extended-spectrumβ-lactamase producing Enterobacteriaceae bacteria

XU Biao; et al

doi:10.13406/j.cnki.cyxb.2004.06.022

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Analysis of drug resistance and genotype of extended-spectrumβ-lactamase producing Enterobacteriaceae bacteria

更新时间：2024-01-17

- Analysis of drug resistance and genotype of extended-spectrumβ-lactamase producing Enterobacteriaceae bacteria
- Journal of Chongqing Medical University Issue 6, Pages: 781-783(2004)
- 作者机构：
  
  1. 重庆医科大学临床学院检验科
  2. 重庆医科大学临床学院检验科,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2004.06.022
  CLC： R446.5
- Published：2004，
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[1]胥飚,曹何,余显书.肠杆菌科产超广谱β-内酰胺酶菌株的耐药性及基因型分析[J].重庆医科大学学报,2004(06):781-783.

XU Biao, et al. Analysis of drug resistance and genotype of extended-spectrumβ-lactamase producing Enterobacteriaceae bacteria. [J]. Journal of Chongqing Medical University (6):781-783(2004)
[1]胥飚,曹何,余显书.肠杆菌科产超广谱β-内酰胺酶菌株的耐药性及基因型分析[J].重庆医科大学学报,2004(06):781-783. DOI： 10.13406/j.cnki.cyxb.2004.06.022.

XU Biao, et al. Analysis of drug resistance and genotype of extended-spectrumβ-lactamase producing Enterobacteriaceae bacteria. [J]. Journal of Chongqing Medical University (6):781-783(2004) DOI： 10.13406/j.cnki.cyxb.2004.06.022.

摘要

目的 :了解我院临床分离的产超广谱β -内酰胺酶（ESBL）的肠杆菌科常见菌的耐药状况及ESBLs的基因型。方法 :对 2 0 0 2年 9月至 2 0 0 3年 10月我院临床分离的 2 5 7株肠杆菌科常见细菌参照美国临床实验室标准化委员会（NCCLS）M10 0-S9文件的确认试验测定ESBLs;将产ESBLs的 6 3株细菌再利用生物梅里埃公司的ATBG - 5药敏卡进行耐药性分析 ;并分别用SHV、TEM、CTX -M型基因的特异性引物进行PCR扩增

以确定ESBLs的基因型。结果 :6 3株产ESBLs菌对亚胺培南、美洛匹林、头孢西丁、头孢吡肟、头孢他啶、阿米卡星、头孢噻肟、环丙沙星的耐药率分别为 :1.6 %、1.6 %、38.1%、39.7%、4 6 .0 %、5 5 .6 %、6 3.5 %、95 .2 % ;携带SHV、TEM、CTX -M型ESBLs的比率分别为 :84 .1%、4 7.6 %、2 5 .4 %。结论 :我院产ESBLs菌的多重耐药现象明显

亚胺培南、美洛匹林仍是治疗产ESBLs菌感染的首选药物 ;SHV型为主要产酶型别

我院存在CTX -M酶的流行。

Abstract

Objective:To investigate the drug resistance and genotypes of extended-spectrum β-lactamase(ESBL)-producing Enterobacteriaceae bacteria in our hospital.Methods:257 strains of Enterobacteriaceae bacteria were isolated from clinical specimens in our hospital from Sept.2002 to Oct.2003.Sixty-three strains of ESBLs-producing isolates were detected by phenotypic confirmatory test according to NCCLS M100-S9.The susceptibility test of them to the antibiotics were detected with Bio-Merieux ATB G-5.The SHV-type

TEM-type

CTX-M-type encoding genes were amplified by PCR.Results:The resistant rates of the 63 strains to IMP

MERO

CXT

FEP

CAZ

AKN

CTX and CIP were 1.6%

1.6%

38.1%

39.7%

46.0%

55.6%

63.5% and 95.2%

repectively.The rates of Enterobacteriaceae bacteria carrying SHV-type

TEM-type

CTX-M-type ESBLs were 84.1%

47.6% and 25.4%

repectively.Conclusion:Multidrug resistance of the ESBLs-producing strains in our hospital is obvious.IMP and MERO are still the best choices for treatment of the infections caused by ESBLs-producing bacteria.Most of the ESBLs-producing strains carry SHV-type.CTX-M-type β-lactamase exists prevalently in our hospital.

关键词

肠杆菌科细菌超广谱β-内酰胺酶耐药基因型

Keywords

EnterobacteriaceaeExtended-spectrum β-lactamasedrug resistancegenotype

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