Effect of TSPG on apoptosis of K562 cells and the expression of Fas,sfas

CAO Weixi; et al

doi:10.13406/j.cnki.cyxb.2004.06.020

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Effect of TSPG on apoptosis of K562 cells and the expression of Fas,sfas

更新时间：2024-01-17

- Effect of TSPG on apoptosis of K562 cells and the expression of Fas,sfas
- Journal of Chongqing Medical University Issue 6, Pages: 774-776(2004)
- 作者机构：
  
  1. 重庆医科大学检验系临床检验诊断学重点实验室
  2. 重庆医科大学检验系临床免疫学教研室
  3. 重庆市中医研究所
  4. 重庆医科大学基础医学院组胚教研室,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2004.06.020
  CLC： R733.7
- Published：2004，
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[1]曹唯希,陈婷梅,刘琼,王亚平,姜蓉.人参总皂甙对K562细胞Fas及其可溶性受体的影响[J].重庆医科大学学报,2004(06):774-776.

CAO Weixi, et al. Effect of TSPG on apoptosis of K562 cells and the expression of Fas,sfas. [J]. Journal of Chongqing Medical University (6):774-776(2004)
[1]曹唯希,陈婷梅,刘琼,王亚平,姜蓉.人参总皂甙对K562细胞Fas及其可溶性受体的影响[J].重庆医科大学学报,2004(06):774-776. DOI： 10.13406/j.cnki.cyxb.2004.06.020.

CAO Weixi, et al. Effect of TSPG on apoptosis of K562 cells and the expression of Fas,sfas. [J]. Journal of Chongqing Medical University (6):774-776(2004) DOI： 10.13406/j.cnki.cyxb.2004.06.020.

摘要

目的 :研究人参总皂甙（TSPG）诱导K5 6 2细胞凋亡的机理。方法 :采用形态学观察、原位末端标记法（TUNEL）观察不同浓度人参总皂甙对K5 6 2白血病细胞凋亡的影响 ;用免疫组化法检测TSPG诱导K5 6 2细胞凋亡中Fas表达情况

并采用酶联免疫吸附法（ELISA）检测可溶性Fas（sFas）。结果 :5 0、10 0 μg/mlTSPG可诱导K5 6 2细胞凋亡 ;同时实验组Fas表达明显增高（P <0 .0 1）

而sfas无明显变化。结论 :TSPG诱导K5 6 2细胞凋亡与其诱导细胞Fas高表达有关。

Abstract

Objective:Our purpose is to clarify the mechanisms for total saponins of Panax ginseng(TSPG) inducing K562 cells to apoptosis.Methods:By using morphological observation and TUNEL

the effect of TSPG on apoptosis of K562 cells were studied.The expression of Fas was studied by immunocytochemistry

and sfas was assayed by ELISA.Results:The results indicated that 50

100μg/ml TSPG could induce K562 cells to apoptosis.Our experiment also showed after induced by TSPG

the ratio of positive K562 cells of expression Fas is increased ( P <0.01)

while sfas had no change.Conclusion:The mechanisms for TSPG inducing K562 cells to apoptosis may be related to the increased expression of Fas in K562 cells.

关键词

人参总皂甙K562凋亡Fassfas

Keywords

TSPGK562apoptosisFassfas

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