Study on apoptosis of human hepatoma cell line HepG2 induced by oxaliplatin

ZHANG Yan; et al

doi:10.13406/j.cnki.cyxb.2004.06.011

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Study on apoptosis of human hepatoma cell line HepG2 induced by oxaliplatin

更新时间：2024-01-17

- Study on apoptosis of human hepatoma cell line HepG2 induced by oxaliplatin
- Journal of Chongqing Medical University Issue 6, Pages: 745-748(2004)
- 作者机构：
  
  1. 重庆医科大学第二临床学院消化内科
  2. 重庆医科大学病理生理教研室,重庆,400010
  3. ,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2004.06.011
  CLC： R735.7
- Published：2004，
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[1]张燕,左国庆,汤为学.奥沙利铂诱导人肝癌细胞株HepG2凋亡及其机制探讨[J].重庆医科大学学报,2004(06):745-748.

ZHANG Yan, et al. Study on apoptosis of human hepatoma cell line HepG2 induced by oxaliplatin. [J]. Journal of Chongqing Medical University (6):745-748(2004)
[1]张燕,左国庆,汤为学.奥沙利铂诱导人肝癌细胞株HepG2凋亡及其机制探讨[J].重庆医科大学学报,2004(06):745-748. DOI： 10.13406/j.cnki.cyxb.2004.06.011.

ZHANG Yan, et al. Study on apoptosis of human hepatoma cell line HepG2 induced by oxaliplatin. [J]. Journal of Chongqing Medical University (6):745-748(2004) DOI： 10.13406/j.cnki.cyxb.2004.06.011.

摘要

目的 :研究奥沙利铂对人肝癌细胞株HepG2生长抑制及诱导凋亡的作用并探讨其诱导凋亡的作用机制。方法 :应用MTT法检测奥沙利铂对HepG2细胞生长的抑制作用 ;电镜观察细胞的形态改变 ;流式细胞仪分析细胞周期的分布和凋亡的情况 ;免疫组织化学法检测突变型p5 3

Bcl- 2

Bax

Fas等凋亡相关基因蛋白的表达。结果 :奥沙利铂对HepG2有明显的抑制作用

细胞阻滞于S期和G2 /M期

G0 /G1期细胞减少

作用 72h出现凋亡峰

电镜可以找到凋亡小体

免疫组织化学表明Bax表达增强

突变型 p5 3、Bcl- 2表达减弱

Fas表达无差异。结论 :奥沙利铂具有抑制肝癌细胞株HepG2增殖及诱导凋亡作用

其机制与促凋亡相关基因表达增强和抗凋亡相关基因表达减弱有关。

Abstract

Objective:To study the effects of oxaliplatin on the proliferation and apoptosis of human hepatoma cell line HepG2 and to investigate its mechanism.Methods:The inhibition of HepG2 cell proliferation was estimated by MTT.Morphologic changes under electron microscope were observed.Alteration of cell cycle and apoptosis were analyzed using flow cytometry.The expression of apoptosis-associated genes mutant type p53

Bcl-2

Bax and Fas were detected with immunohistochemical technique.Results:Oxaliplatin could apparently inhibit the growth of HepG2 cells.Cell cycle analysis indicated that oxaliplatin blocked cells in S and G 2/M phase and the apoptosis emerged when treated with oxaliplatin for 72 hours.Under transmission electron microscope

apoptotic body could be found.The expression of genes Bax was up-regulated

the expression of genes mutant type p53

Bcl-2 was down-regulated

while the expression of Fas was not changed.Conclusion:Oxaliplatin can inhibit the proliferation and induce apoptosis of HepG2 cells.The apoptosis is related to the up-regulation apoptotic genes and down-regulation of anti-apoptotic genes.

关键词

奥沙利铂肝细胞癌细胞周期凋亡

Keywords

oxaliplatinhepatocellular carcinomacell cycleapoptosis

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