RNA interfering on the cell cycle of gastric cancer by the recombinantplasmid affecting DNMT1 gene expression

CHEN Daorong; et al

doi:10.13406/j.cnki.cyxb.2004.06.009

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RNA interfering on the cell cycle of gastric cancer by the recombinantplasmid affecting DNMT1 gene expression

更新时间：2024-01-17

- RNA interfering on the cell cycle of gastric cancer by the recombinantplasmid affecting DNMT1 gene expression
- Journal of Chongqing Medical University Issue 6, Pages: 738-741(2004)
- 作者机构：
  
  1. 重庆医科大学临床学院消化内科
  2. 重庆医科大学病毒性肝炎研究所,重庆,400016
  3. ,重庆,400010
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2004.06.009
  CLC： R735.2
- Published：2004，
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[1]陈道荣,王丕龙,黄爱龙.DNMT1靶向RNAi重组体对胃癌细胞周期的影响[J].重庆医科大学学报,2004(06):738-741.

CHEN Daorong, et al. RNA interfering on the cell cycle of gastric cancer by the recombinantplasmid affecting DNMT1 gene expression. [J]. Journal of Chongqing Medical University (6):738-741(2004)
[1]陈道荣,王丕龙,黄爱龙.DNMT1靶向RNAi重组体对胃癌细胞周期的影响[J].重庆医科大学学报,2004(06):738-741. DOI： 10.13406/j.cnki.cyxb.2004.06.009.

CHEN Daorong, et al. RNA interfering on the cell cycle of gastric cancer by the recombinantplasmid affecting DNMT1 gene expression. [J]. Journal of Chongqing Medical University (6):738-741(2004) DOI： 10.13406/j.cnki.cyxb.2004.06.009.

摘要

目的 :本研究利用RNA干扰（RNAinterfering

RNAi）技术

以DNMT1（DNAmethyltransferase 1）为靶基因

设计构建重组体

研究其对胃癌细胞周期的影响。方法 :设计有小发夹结构的两条DNA序列

经退火成互补双链

再克隆至载体pTZU6 +1中构建重组体

经鉴定正确后

转染胃癌细胞AGS

最后使用流式细胞仪分析细胞周期。结果 :转染后的胃癌细胞的S期细胞较前有明显减少

G2 -M期细胞明显增多。结论 :DNMT1靶向RNA干扰重组体转染到胃癌细胞中后促使S期细胞进入G2 -M期

最后衰老死亡。从而为肿瘤的治疗开辟了新途径。

Abstract

Objective:To clone the recombinant plasmid affecting gene DNMT1 expression by RNA interfering and transfect it into AGS in order to further search its effects on the cell cycle of gastric cancer AGS.Methods:Two DNA sequences containing short hairpin structure was designed and synthesized.The complement form was obtained by annealing and inserted into vector pTZU6+1.The recombinant plasmid was transfected into AGS.Finally the cell cycle was evaluated through flow cytometer.Results:The S cycle cells of the transfected AGS dropped dramatically.And the G2-M cycle cells increased significantly.Conclusion:Successful cloning and transfecting the recombinant make it possible to search new gene therapy for the tumors.

关键词

DNMT1RNA干扰胃癌细胞细胞周期

Keywords

DNMT1RNA interferingGastric cancercell cycle

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