Study on suitable isolation and culture of mesenchymal stem cell of rats in vitro

LU Tiewei; et al

doi:10.13406/j.cnki.cyxb.2004.06.003

您当前的位置：

首页 >

文章列表页 >

Study on suitable isolation and culture of mesenchymal stem cell of rats in vitro

更新时间：2024-01-17

- Study on suitable isolation and culture of mesenchymal stem cell of rats in vitro
- Journal of Chongqing Medical University Issue 6, Pages: 715-717(2004)
- 作者机构：
  
  1. 重庆医科大学儿科学院心内科
  2. 重庆医科大学儿科研究所,重庆,400014
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2004.06.003
  CLC： R329
- Published：2004，
扫描看全文
[1]吕铁伟,田杰,朱静,刘官信.大鼠骨髓间充质干细胞适宜分离和培养条件的研究[J].重庆医科大学学报,2004(06):715-717+721.

LU Tiewei, et al. Study on suitable isolation and culture of mesenchymal stem cell of rats in vitro. [J]. Journal of Chongqing Medical University (6):715-717(2004)
[1]吕铁伟,田杰,朱静,刘官信.大鼠骨髓间充质干细胞适宜分离和培养条件的研究[J].重庆医科大学学报,2004(06):715-717+721. DOI： 10.13406/j.cnki.cyxb.2004.06.003.

LU Tiewei, et al. Study on suitable isolation and culture of mesenchymal stem cell of rats in vitro. [J]. Journal of Chongqing Medical University (6):715-717(2004) DOI： 10.13406/j.cnki.cyxb.2004.06.003.

摘要

目的 :寻求适宜有效的大鼠骨髓间充质干细胞（mensechymalstemcell

MSCs）体外分离、纯化和培养的条件

为MSCs进一步的实验研究打下基础。方法 :分别用贴壁筛选法和密度梯度离心法来分离和纯化MSCs

并用不同的接种密度、不同的血清浓度和不同的培养基检测细胞的生长情况

绘制出细胞的生长曲线 ;流式细胞仪检测MSCs生长周期。结果 :细胞贴壁呈纤维状生长

以 2× 10 9的浓度接种

在含 10 %胎牛血清的DMEM /F12培养基中

37℃、5 %CO2 细胞培养箱中

细胞生长状况良好 ;细胞在培养的最初几天处于生长的潜伏期

而后呈指数级生长

随着时间的延伸

细胞的生长速度减慢 ;而流式细胞仪检测示细胞大多处于G0 G1期。结论 :在适宜的培养条件下

MSCs在体外培养的过程中生长状态良好

增殖速度快

为进一步的研究打下了基础。

Abstract

Objective:To explore the method of isolation

purification and culture of rat mesenchymal stem cells (MSCs) in vitro in order to provide an experimental basis for further study.Methods:MSCs were acquired by adhesive screening and density gradient centrifugation methods.The growth of MSCs at different plate densities

different serum concentrations and different culture media were observed and their growth curves were charted.The cell cycle was detected by flow cytometer.Results:The adherent and fibroblast-shaped cells were in good growth state

which were plated at 2×10 9/ml and cultured in DMEM/F12 medium supplemented with 10% fetal bovine serum and placed in 37℃

5%CO 2 incubator.Cells grew slowly the first several days after plated

then the growth accelerated at index speed.The results of the cell cycle detection demonstrated that most of MSCs were in G 0G 1 phase.Conclusion:In suitable conditions

MSCs show stable growth and rapid proliferation in vitro.

关键词

骨髓间充质干细胞细胞培养大鼠

Keywords

mesenchymal stem cellcell culturerat

references

Views

171

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Histopathological changes and pathological basis of the cryptorchidtestis in rats by maternal exposure to flutamide

DISPOSITION OF ~3H-PIPERAZINE FERULATE IN RATS

MICROENCAPSULATION OF ISLETS OF LANGERHANS Ⅱ . THE INSULIN SECRETION OF THE MICROENCAPSULATED RAT’S ISLETS DURING IN VITRO CULTURE USING CO2 AND COMMON INCUBATOR

PHA INDUCED RAT T-LYMPHOCYTE TRANSFOR MATION IN VIVO

THE ALTERATIONS OF RENAL AND HEMODYNAMIC EFFECTS OF NATRIURETIC FACTOR IN ATRIA OF THE RATS WITH ACUTE HEART FAILURE

Related Author

Peng Jinpu

Zhang Deying

Cao Xining

Zhou Yue

Shen Lianju

Long Chunlan

He Dawei

Lin Tao

Related Institution

Department of Urology,the Children’s Hospital,Chongqing Medical University,Ministry of Education Key Laboratory of Child Development and Disorders,Key Laboratory of Pediatrics in Chongqing,Chongqing International Science and Technology Cooperation Center for Child Development and Disorders

重庆医科大学第一医院临床药理研究室

重庆医科大学基础医学部药理教研室

Tang Wengan

重庆医科大学人工肝研究室

⁰