Expression of Toll like receptor 4 and MD-2 gene and protein in Kupffer cellsfollowing ischemia-reperfusion injury in rat liver transplant

PENG Yong; et al

doi:10.13406/j.cnki.cyxb.2004.06.002

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Expression of Toll like receptor 4 and MD-2 gene and protein in Kupffer cellsfollowing ischemia-reperfusion injury in rat liver transplant

更新时间：2024-01-17

- Expression of Toll like receptor 4 and MD-2 gene and protein in Kupffer cellsfollowing ischemia-reperfusion injury in rat liver transplant
- Journal of Chongqing Medical University Issue 6, Pages: 711-714(2004)
- 作者机构：
  
  1. 重庆医科大学第二临床学院肝胆外科
  2. 重庆医科大学第二临床学院肝胆外科,重庆,400010
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2004.06.002
  CLC： R657.3
- Published：2004，
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[1]彭勇,龚建平,刘长安,刘海忠,李旭宏,李寿柏.大鼠肝移植缺血再灌注后Kupffer细胞Toll样受体4及其伴侣分子MD-2的表达[J].重庆医科大学学报,2004(06):711-714.

PENG Yong, et al. Expression of Toll like receptor 4 and MD-2 gene and protein in Kupffer cellsfollowing ischemia-reperfusion injury in rat liver transplant. [J]. Journal of Chongqing Medical University (6):711-714(2004)
[1]彭勇,龚建平,刘长安,刘海忠,李旭宏,李寿柏.大鼠肝移植缺血再灌注后Kupffer细胞Toll样受体4及其伴侣分子MD-2的表达[J].重庆医科大学学报,2004(06):711-714. DOI： 10.13406/j.cnki.cyxb.2004.06.002.

PENG Yong, et al. Expression of Toll like receptor 4 and MD-2 gene and protein in Kupffer cellsfollowing ischemia-reperfusion injury in rat liver transplant. [J]. Journal of Chongqing Medical University (6):711-714(2004) DOI： 10.13406/j.cnki.cyxb.2004.06.002.

摘要

目的 :研究大鼠肝移植缺血再灌注后Kupffer细胞Toll样受体 4 （TLR4 ）及MD - 2的表达

并探讨其在再灌注损伤中的作用。方法 :分离培养大鼠肝移植缺血再灌注后 0 （对照组）、2、12、2 4h（再灌注组）的Kupffer细胞

检测TLR4 /MD - 2mRNA、蛋白以及上清TNF -α的表达

并在培养液中加入抗TLR4抗体（抗TLR4组）

观察TLR4抗体对TNF -α分泌的影响。结果 :再灌注后Kupffer细胞TLR4 /MD - 2mRNA、蛋白以及TNF -α表达较对照组明显升高（P <0 .0 1）

应用抗TLR4抗体后

TNF -α量较再灌注组明显降低（P <0 .0 1）。结论 :TLR4及其伴侣分子MD - 2在介导Kupffer细胞激活和肝移植缺血再灌注损伤中可能起重要作用。

Abstract

Objective:To study the expression of Toll like receptor 4(TLR4) and MD-2 gene and protein in Kupffer cells and its role in ischemia-reperfusion injury (IRI) on rat liver graft.Methods:The Kupffer cells were isolated at 0(control group)

24h (IR group)following IR.The mRNA and protein expressions of TLR4 and MD-2 were detected by RT-PCR and flow cytometry (FCM)

respectively.The TNF-α level in supernatant was measured by ELISA.Then the isolated KCs were incubated with anti-TLR4 polyclonal antibody (anti-TLR4 group)

and the TNF-α level were measured again.Results: The mRNA and protein expressions of TLR4/MD-2 and the level of TNF-α in IR group increased significantly following IR compared with those in control group( P <0.01).The TNF-α production was greatly inhibted after anti-TLR4 antibody treatment (compared with IR group

P <0.01).Conclusion:TLR4 and its partner molecule MD-2 may take an important part in the Kupffer cell activation and IRI.

关键词

肝移植缺血再灌注损伤Toll样受体4Kupffer细胞

Keywords

liver transplantationischemia-reperfusion injuryToll like receptor 4Kupffer cell

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