An experimental investigation on sodium hyaluronate in mid-Term cornea preservation

YANG Juan; et al

doi:10.13406/j.cnki.cyxb.2004.05.031

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An experimental investigation on sodium hyaluronate in mid-Term cornea preservation

更新时间：2024-01-17

- An experimental investigation on sodium hyaluronate in mid-Term cornea preservation
- Journal of Chongqing Medical University Issue 5, Pages: 664-667(2004)
- 作者机构：
  
  1. 重庆医科大学临床学院眼科
  2. 重庆医科大学临床学院眼科,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2004.05.031
  CLC： R779.65
- Published：2004，
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[1]杨娟,赵敏,徐智勇,李鸿.透明质酸钠在角膜中期保存中的实验研究[J].重庆医科大学学报,2004(05):664-667.

YANG Juan, et al. An experimental investigation on sodium hyaluronate in mid-Term cornea preservation. [J]. Journal of Chongqing Medical University (5):664-667(2004)
[1]杨娟,赵敏,徐智勇,李鸿.透明质酸钠在角膜中期保存中的实验研究[J].重庆医科大学学报,2004(05):664-667. DOI： 10.13406/j.cnki.cyxb.2004.05.031.

YANG Juan, et al. An experimental investigation on sodium hyaluronate in mid-Term cornea preservation. [J]. Journal of Chongqing Medical University (5):664-667(2004) DOI： 10.13406/j.cnki.cyxb.2004.05.031.

摘要

目的 :研究透明质酸钠（SH）在角膜中期保存中的作用。方法 :在中期保存液（MMK液）中

加入SH制成保存液Ⅰ、Ⅱ、Ⅲ （浓度分别为 0 .0 5 %、0 .0 3%、0 .0 1% ）

以未加SH的MMK液作对照并作组间对照。对保存后的兔角膜片行大体和超微结构观察、内皮细胞苔盼兰 -茜素红染色、琥珀酸脱氢酶（SDH）、葡萄糖 - 6 -磷酸酶（G - 6 -P）活性检测。结果 :加入SH的三组所保存的角膜片其透明度、超微结构均好于对照组

角膜内皮细胞活性以实验Ⅰ组最高

对照组最低

各组间比较有显著性差异。结论 :SH对角膜内皮细胞有明显的保护作用

可提高保存角膜的质量

延长保存时间

其效果与SH浓度有关

Abstract

Objective:To study the action of SH in mid-term cornea preservation.Methods:SH was added into mid-term preservation solution(MMK) to make the new storage media with SH of three different concentration (0.05%

0.03%

0.01%).The contrast group was MMK medium without SH.The corneal appearance

transparence and ultrastructure were observed

and trypan blue-alizarin red staining of CEC

SDH and G-6-P enzyme staining with histochemistry were made in different storage time.Results:The transparence of cornea and ultrastructure of CEC in all the experimental groups were better than that of the contrast.The activity degree of CEC was highest in group I

lowest in the contrast

and statistically significant among all the groups.Conclusion:SH plays an important role in protecting CEC in middle storage medium

and enhances the quality and prolongs the duration of the preserved corneas.The protective effect is determined by the consistence of SH.

关键词

透明质酸钠内皮细胞角膜保存眼库技术

Keywords

sodium hyaluronateCorneal endothelial cellsCornea preservationEyebank technique

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