Therapeutic effect of adefovir anti-duck hepatitis B virus in vivo and anti-HBV activity in vitro (2.2.15 cells)

CHEN Yaxi; et al

doi:10.13406/j.cnki.cyxb.2004.05.017

您当前的位置：

首页 >

文章列表页 >

Therapeutic effect of adefovir anti-duck hepatitis B virus in vivo and anti-HBV activity in vitro (2.2.15 cells)

更新时间：2024-01-17

- Therapeutic effect of adefovir anti-duck hepatitis B virus in vivo and anti-HBV activity in vitro (2.2.15 cells)
- Journal of Chongqing Medical University Issue 5, Pages: 618-621(2004)
- 作者机构：
  
  1. 重庆医科大学病毒性肝炎研究所
  2. 重庆医科大学病毒性肝炎研究所,重庆,400010
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2004.05.017
  CLC： R96
- Published：2004，
扫描看全文
[1]陈压西,黄爱龙,郭晖,齐珍元.阿的福韦在鸭乙型肝炎模型及2.2.15细胞中抗乙型肝炎病毒的药效研究[J].重庆医科大学学报,2004(05):618-621.

CHEN Yaxi, et al. Therapeutic effect of adefovir anti-duck hepatitis B virus in vivo and anti-HBV activity in vitro (2.2.15 cells). [J]. Journal of Chongqing Medical University (5):618-621(2004)
[1]陈压西,黄爱龙,郭晖,齐珍元.阿的福韦在鸭乙型肝炎模型及2.2.15细胞中抗乙型肝炎病毒的药效研究[J].重庆医科大学学报,2004(05):618-621. DOI： 10.13406/j.cnki.cyxb.2004.05.017.

CHEN Yaxi, et al. Therapeutic effect of adefovir anti-duck hepatitis B virus in vivo and anti-HBV activity in vitro (2.2.15 cells). [J]. Journal of Chongqing Medical University (5):618-621(2004) DOI： 10.13406/j.cnki.cyxb.2004.05.017.

摘要

目的 :观察阿的福韦体内抗鸭乙型肝炎病毒（DHBV）的作用及体外抗乙型肝炎病毒（HBV）效果。方法 :采用鸭乙型肝炎动物模型

用阿的福韦口服治疗 10天

检测用药前后血清中的DHBVDNA、DHBsAg

并取肝脏样本提取总DNA

作SouthernBlot分析。此外

以 2 .2 .15细胞为靶细胞

给药后通过检测细胞培养液中HBsAg、HBeAg和HBVDNA观察阿的福韦抗HBV效果。结果 :阿的福韦各剂量组用药后均能使血清中的DHBVDNA显著降低或极显著降低（P <0 .0 5或P <0 .0 1）

但停药后有DHBVDNA回升现象 ;用药前后DHBsAg无明显改变。肝组织SouthernBlot分析亦显示用药后肝脏中DHBVDNA有明显降低

停药后DHBVDNA反跳明显。大剂量阿的福韦加入细胞培养 12天

对HBsAg抑制率为 17.0 1%

对HBeAg抑制率为 2 6 .80 %

对HBVDNA抑制率为 2 6 .92 %。结论 :阿的福韦在鸭体内有抗鸭乙型肝炎病毒的作用

但停药后DHBVDNA有“反跳”现象 ;体外实验无明显抗HBV病毒作用

Abstract

Objective:To study the therapeutic effect of the purine nucleoside analog adefovir against duck hepatitis virus (DHBV) in vivo and anti-HBV activity in vitro.Methods:The Chongqing duck hepatitis B animal model was treated with adefovir by intragastric administration once a day for 10 days.DHBV DNA in serum and liver were monitored by serum dot-blot hybridization and Southern blot.DHBsAg in serum was detected simultaneously by ELISA.The anti-HBV activity of adefovir was observed in 2.2.15 cells (human hepatocellular carcinoma cells stably transfected with HBV).Results:Adefovir could significantly reduce DHBV DNA level.After stopping the treatment for 3 days

DHBV DNA level in serum and liver returned significantly.The O.D values of DHBsAg in duck serum did not change at the period of the treatment.After 12 days of continuous exposure to adefovir at the concentration of 20μg/ml in vitro culture

the inhibitions of HBsAg

HBeAg

HBV DNA were 17.01%

26.80%

and 26.92% respectively.Conclusion:The study confirms the safety and potent dose-dependent antihepadnaviral activity of adefovir in vivo.The anti-HBV effect is not demonstrated in 2.2.15 cells.

关键词

阿的福韦鸭乙型肝炎病毒动物模型2.2.15细胞

Keywords

AdefovirDuck hepatitis B virusanimal model2.2.15 cells

references

Views

186

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Experimental study on the effect of Guangxi phyllanthus urinaria on anti-duck hepatitis B virus

A mouse model of polycystic ovary syndrome with abnormal glucose and lipid metabolism induced by dihydrotestosterone combined with high-fat diet

Etablishment and evaluation standards of skin photoaging model in SD rat

Animal model of arteriovenous fistula combined with chronic renal failure in New Zealand white rabbits

Establishment of Chinese rural dog intrauterine adhesion model by mechanical and infection double injury

Related Author

CHEN Ya-xi

et al

齐珍元

张定凤

Wu Jinlin

Zhang Binghan

Long Jiangchuan

Fang Ronghua

Related Institution

Institute For Viral Hepatitis

Department of Endocrinology，Chongqing Traditional Chinese Medicine Hospital

Department of Endocrinology，The Second Affiliated Hospital of Chongqing Medical University

Department of Dermatology,The First Affiliated Hospital of Chongqing Medical University

Department of Vascular Surgery,The First Affiliated Hospital of Chongqing Medical University

⁰