Comparison on cellular immunity against Mycobacterium tuberculosis after intragastric and subcutaneous immunization of BCG in mice

WEI Jiang; et al

doi:10.13406/j.cnki.cyxb.2004.05.013

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Comparison on cellular immunity against Mycobacterium tuberculosis after intragastric and subcutaneous immunization of BCG in mice

更新时间：2024-01-17

- Comparison on cellular immunity against Mycobacterium tuberculosis after intragastric and subcutaneous immunization of BCG in mice
- Journal of Chongqing Medical University Issue 5, Pages: 605-607(2004)
- 作者机构：
  
  1. 重庆医科大学检验系临床免疫学教研室
  2. 重庆医科大学检验系临床免疫学教研室,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2004.05.013
  CLC： R392
- Published：2004，
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[1]魏江,卢贤瑜,陈淑惠,刘明方.小鼠胃内和皮内接种BCG免疫后抗结核细胞免疫的比较[J].重庆医科大学学报,2004(05):605-607.

WEI Jiang, et al. Comparison on cellular immunity against Mycobacterium tuberculosis after intragastric and subcutaneous immunization of BCG in mice. [J]. Journal of Chongqing Medical University (5):605-607(2004)
[1]魏江,卢贤瑜,陈淑惠,刘明方.小鼠胃内和皮内接种BCG免疫后抗结核细胞免疫的比较[J].重庆医科大学学报,2004(05):605-607. DOI： 10.13406/j.cnki.cyxb.2004.05.013.

WEI Jiang, et al. Comparison on cellular immunity against Mycobacterium tuberculosis after intragastric and subcutaneous immunization of BCG in mice. [J]. Journal of Chongqing Medical University (5):605-607(2004) DOI： 10.13406/j.cnki.cyxb.2004.05.013.

摘要

目的 :为比较小鼠胃内与皮内接种BCG后

机体产生抗结核杆菌的细胞免疫。方法 :分别于小鼠胃内、皮内免疫接种BCG的第 6 0、90天

检测小鼠脾淋巴细胞受到PPD刺激后转化率、小鼠脾淋巴细胞受到PPD刺激后IL - 2表达量。结果 :淋巴细胞的转化率分别为 31.30 %

37.90 % （与皮内接种无显著性差异

与未免疫组有显著性差异） ;脾淋巴细胞IL - 2表达量分别为 37.4 7± 5 .6 0 （U/ml）

39.4 1± 7.73（U/ml）（与皮内接种无显著性差异

与未免疫组有显著性差异）。结论 :胃内接种BCG可以产生与皮内接种相近的抗结核细胞免疫

Abstract

Objective:To compare on the cellular immunity against Mycobacterium tuberculosis(MTB)after intragastric and subcutaneous immunization of BCG in mice.Methods:The proliferation of T-lymphocyte with the stimulus of the purified protein derivative(PPD) was measured at day 60 and 90 after intragastric and subcutaneous immunization of BCG in mice.The interleukin-2(IL-2) expression of T-lymphocyte in spleen was detected at day 60 and 90 after intragastric and subcutaneous immunization of BCG.Results:The ratio of T-lymphocyte proliferation was 31.30% and 37.90% respectively.The production of IL-2 was 37.47±5.60(U/ml) and 39.41±7.73(U/ml)respectively.There were no statistical significant differences in the cellular immunity between intragastric and subcutaneous routes( P >0.05).There were statistical significant differences in the cellular immunity between the immunization and no-immunization group( P <0.01).Conclusion:Intragastric and subcutaneous immunization of BCG can induce cellular immunity against Mycobacterium tuberculosis in mice.

关键词

胃内免疫皮内免疫BCG细胞免疫

Keywords

Intragastric immunizationSubcutaneous immunizationBCGcellular immunity

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Related Author

阳强

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卢贤瑜

LI Hui

Li Shuiying

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Related Institution

Department of Clinical Immunology,Key Laboratory of Clinical Medical Diagnosis,Ministry of Education,Chongqing Medical University

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重庆医科大学第一医院传染科

Department of Clinical Immunology, Faculty of Laboratory Medicine

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