Establishment of multidrug resistant hepatoma cell subline  and its biological features

LUO Shiqiao; et al

doi:10.13406/j.cnki.cyxb.2004.05.012

您当前的位置：

首页 >

文章列表页 >

Establishment of multidrug resistant hepatoma cell subline and its biological features

更新时间：2024-01-17

- Establishment of multidrug resistant hepatoma cell subline and its biological features
- Journal of Chongqing Medical University Issue 5, Pages: 601-604(2004)
- 作者机构：
  
  1. 重庆医科大学临床学院普外科
  2. 重庆医科大学临床学院普外科,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2004.05.012
  CLC： R735.71
- Published：2004，
扫描看全文
[1]罗诗樵,罗亿治.人肝肿瘤耐药细胞亚系建立及生物学特性研究[J].重庆医科大学学报,2004(05):601-604.

LUO Shiqiao, et al. Establishment of multidrug resistant hepatoma cell subline and its biological features. [J]. Journal of Chongqing Medical University (5):601-604(2004)
[1]罗诗樵,罗亿治.人肝肿瘤耐药细胞亚系建立及生物学特性研究[J].重庆医科大学学报,2004(05):601-604. DOI： 10.13406/j.cnki.cyxb.2004.05.012.

LUO Shiqiao, et al. Establishment of multidrug resistant hepatoma cell subline and its biological features. [J]. Journal of Chongqing Medical University (5):601-604(2004) DOI： 10.13406/j.cnki.cyxb.2004.05.012.

摘要

目的 :以阿霉素（DOX）诱导建立人肝肿瘤细胞系HepG2的多药耐药亚系HepG2 /DOX

并对HepG2 /DOX的生物学特性及耐药机制进行研究。方法 :以浓度梯度递增方法

用DOX诱导HepG2产生耐药性。显微镜观察细胞形态

绘制生长曲线

作染色体常规及G带分析。MTT法检测HepG2、HepG2 /DOX细胞对抗癌药物的敏感性。应用免疫组化法检测MDR - 1基因产物PgP的表达。结果 :HepG2 /DOX与HepG2在形态上染色体数目和结构均有差异。细胞可在DOX浓度为 0 .1μg/ml培养基内生长

但生长速度减慢。DOX、顺铂（CDDP）、丝裂霉素（MMC）、氟脲嘧啶（5 -FU）、氨甲喋吟（MTX）对HepG2、HepG2 /DOX细胞的IC5 0值（μg/ml）分别为（0 .0 70和 1.16 1）、（0 .2 14和 1.317）、（0 .16 2和 0 .4 98）、（0 .313和 16 83）、（0 .0 0 7和 0 .2 17）。HepG2 /DOX细胞PgP表达增高。在无DOX培养基内生长 5周后

DOX对HepG2 /DOX的IC5 0值（μg/ml）为 0 .6 84。结论 :HepG2 /DOX有多药耐药的特点

其耐药性与Pgp的表达增高及倍增时间延长有关

Abstract

Objective:To establish multidrug resistant human hepatoma cell subline(HepG2/DOX) and study its biological features.Methods:Doxorubincin-resistant human hepatoma cell subline (HepG2/DOX) was induced by doxorubincin-gradually increasing concentration.To observe cells by light microscope and analyze number and structure of chromosomes of these tumor cells.Sensitivity of anticancer drugs was screened in HepG2

HepG2/DOX cells by MTT method.The immunohistochemistry method was used to measure MDR-1 gene product Pgp expression.Results:HepG2/DOX and HepG2 were different in shape

growth speed.The number and structure of their chromosomes were also different.The value of 50% inhibitory concentration(IC50

μg/ml) for DOX

CDDP

MMC

5-FU

and MTX in HepG2

HepG2/DOX cells was (0.070 and 1.161)

(0.214 and 1.317)

(0.162 and 0.498)

(0.313 and 1.683)

007 and 0.217) respectively.MDR-l gene product Pgp170 expression was found either in HepG2/DOX or HepG2 cells.Pgp170 exprssion in HepG2/DOX was higher than in HepG2.Once HepG2/DOX had been cultured in DOX free RPMl1640 medium for 5 weeks

the IC50(μg/ml)for DOX in HepG2/DOX reduced to 0.684.Conclusion:HepG2/DOX has the characteristics of MDR.The drug-resistance was correlated with over expression of MDR-1 gene product Pgp 170 and slow growth.

关键词

肝肿瘤多药耐药细胞系

Keywords

Liver neoplasmamultidrug resistanceCell line

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

The influence of MDR1 gene therapy on the survival rate of few late hepatocarcinoma balb/c mouse

The expression of P-gp during the MDR1 gene transferring into the hematopoietic cells of the Balb/c mouse bone marrow

Investigation of the effect of verapamil and chemotherapeutic agents on the P-glucoprotein of the KBV200 cell line

Expression characteristics of 5 drug-resistance genes in 6 common adult malignant tumors

Reversal effect of tetrandrine on multidrug resistance of the KBV200 cell line

Related Author

王珊

安淑华

康权

et al

GUO Chunbao

王珊

安淑华

康权

Related Institution

Department of Surgery,College of Pediatrics,Chongqing Medical University

重庆医科大学儿科学院外科

Department of Surgery,College of Pediatrics,Chongqing Medical University

重庆医科大学儿科学院普外科

Department of Otolaryngology,the First Affilated Hospital,Chongqing Medical University

⁰