The (CCTTT)n microsatellite polymorphism of NOS2 gene in Han nationality in the southwest of China

ZHANG Zheng; et al

doi:10.13406/j.cnki.cyxb.2004.05.009

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The (CCTTT)n microsatellite polymorphism of NOS2 gene in Han nationality in the southwest of China

更新时间：2024-01-17

- The (CCTTT)n microsatellite polymorphism of NOS2 gene in Han nationality in the southwest of China
- Journal of Chongqing Medical University Issue 5, Pages: 591-593(2004)
- 作者机构：
  
  1. 重庆医科大学基础医学院生物学教研室
  2. 重庆医科大学基础医学院生物学教研室,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2004.05.009
  CLC： Q987
- Published：2004，
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[1]张政,彭惠民,郭风劲,晏宁.中国西南地区汉族人群NOS2基因(CCTTT)n微卫星多态性研究[J].重庆医科大学学报,2004(05):591-593+611.

ZHANG Zheng, et al. The (CCTTT)n microsatellite polymorphism of NOS2 gene in Han nationality in the southwest of China. [J]. Journal of Chongqing Medical University (5):591-593(2004)
[1]张政,彭惠民,郭风劲,晏宁.中国西南地区汉族人群NOS2基因(CCTTT)n微卫星多态性研究[J].重庆医科大学学报,2004(05):591-593+611. DOI： 10.13406/j.cnki.cyxb.2004.05.009.

ZHANG Zheng, et al. The (CCTTT)n microsatellite polymorphism of NOS2 gene in Han nationality in the southwest of China. [J]. Journal of Chongqing Medical University (5):591-593(2004) DOI： 10.13406/j.cnki.cyxb.2004.05.009.

摘要

目的 :探讨NOS2基因（CCTTT）n微卫星多态性在中国西南地区汉族人群中的分布

并比较不同种族及同一种族但不同地域人群基因频率的差异。方法 :柱层析法提取血细胞基因组DNA

应用PCR、PAGE及银染和DNA测序技术

对 2 0 5例中国西南地区汉族人进行了NOS2基因多态性分析。结果 :（1）共检出 9种等位基因和 30种基因型。（2 ）（CCTTT） 9、（CCTTT） 10 、（CCTTT） 12 、（CCTTT） 13 等位基因频率在中国西南地区与华北地区汉族人群间差异有显著性。（3）（CCTTT） 9、（CCTTT） 10 、（CCTTT） 11、（CCTTT） 15、（CCTTT） 16等位基因频率在中国西南地区汉族人群与英国高加索人群间差异有显著性。结论 :NOS2基因（CCTTT）n微卫星多态性在不同种族间存在差异

在同一种族不同地区也存在着差异

进一步为研究NOS2基因多态性与疾病的关系提供了基础资料

Abstract

Objective:To investigate genotypes and alleles’ distributions of inducible nitric oxide synthase (NOS2) gene (CCTTT)n polymorphism in Han nationality in the southwest of China

and make comparisons with different race or the same race in different areas.Methods:The (CCTTT)n polymorphisms of NOS2 gene were analyzed in a total of 205 southwestern Chinese healthy subjects by polymerase chain reaction (PCR)-8% denature polyacrylamide gel electrophoresis(PAGE)-silver staining and DNA sequencing.Results:(1)Nine alleles and thirty genotypes were identified.(2)(CCTTT) 9

(CCTTT) 10

(CCTTT) 12

(CCTTT) 13 alleles frequencies of NOS2 gene in Chinese Han population in southwestern area differed significantly from those in northern area.(3)(CCTTT) 9

(CCTTT) 10

(CCTTT) 11

(CCTTT) 15

(CCTTT) 16 alleles frequencies of NOS2 gene in Han population of the southwest of China differed significantly from those of Caucasians in England.Conclusion:The (CCTTT)n microsatellite polymorphisms of NOS2 gene are different not only among different ethnic populations but also within the same population of different areas

which may be of significance in the studies on relationship between genetics and disease.

关键词

一氧化氮合酶基因多态性聚合酶链反应

Keywords

Nitric oxide synthasegenetic polymorphismPCR

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