Effects of environment in vivo and in vitro on the differentiation of bone mesenchymal stem cells into neural cells

ZHUO Benhui; et al

doi:10.13406/j.cnki.cyxb.2004.05.008

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Effects of environment in vivo and in vitro on the differentiation of bone mesenchymal stem cells into neural cells

更新时间：2024-01-17

- Effects of environment in vivo and in vitro on the differentiation of bone mesenchymal stem cells into neural cells
- Journal of Chongqing Medical University Issue 5, Pages: 588-590(2004)
- 作者机构：
  
  1. 重庆医科大学儿科学院儿保科
  2. 重庆医科大学临床学院神内科
  3. 重庆医科大学儿科学院儿保科,重庆,400014
  4. ,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2004.05.008
  CLC： R329.2
- Published：2004，
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[1]卓本慧,万东,江和碧,罗勇,李廷玉.体内外环境对骨髓间充质干细胞向神经细胞分化的影响[J].重庆医科大学学报,2004(05):588-590+628.

ZHUO Benhui, et al. Effects of environment in vivo and in vitro on the differentiation of bone mesenchymal stem cells into neural cells. [J]. Journal of Chongqing Medical University (5):588-590(2004)
[1]卓本慧,万东,江和碧,罗勇,李廷玉.体内外环境对骨髓间充质干细胞向神经细胞分化的影响[J].重庆医科大学学报,2004(05):588-590+628. DOI： 10.13406/j.cnki.cyxb.2004.05.008.

ZHUO Benhui, et al. Effects of environment in vivo and in vitro on the differentiation of bone mesenchymal stem cells into neural cells. [J]. Journal of Chongqing Medical University (5):588-590(2004) DOI： 10.13406/j.cnki.cyxb.2004.05.008.

摘要

目的 :探讨体内外环境对骨髓间充质干细胞（MSCs）向神经细胞分化的影响。方法 :①DAPI体外标记MSCs后

直接注入大鼠大脑中动脉梗塞（MACO）模型（n =6 ）的脑内

2周后取脑组织

用免疫荧光检测MSCs来源细胞的巢蛋白（nestin）、微管相关蛋白 - 2 （MAP - 2 ）、胶质纤维酸性蛋白（GFAP）的表达。②体外用全反式视黄酸（ATRA）预诱导 2 4h后

换用改良的神经细胞培养基（MNM ）培养 18h

免疫组化检测nestin、MAP - 2、GFAP的表达。结果 :①植入 2周后

MSCs在注射部位及其周围广泛分布

仅有少许细胞表达nestin、MAP - 2和GFAP。②MSCs在体外诱导后

nestin、MAP - 2的阳性率分别为（92 .3± 3.4 ） %和（89.6 +3.3） %

但不表达GFAP。结论 :MSCs在脑内向神经细胞转化的转化率较低

而在体外转化为神经元的转化率相当高

体内外环境对MSCs向神经细胞的分化的过程及机理存在较大差异。

Abstract

Objective:To investigate the effects of environment in vitro and in vivo on the differentiation of bone mesenchymal stem cells (MSCs) into neural cells.Methods:①MSCs were labeled with 4

6-diamidino-2-phenylindole (DAPI) and injected into the right brain of the rats(n=6)

which had suffered from middle cerebral artery occlusion(MACO) a day before.Two weeks later

immunofluorescent staining was used to detect the expression of nestin

microtubule-associated protein 2 (MAP-2) and glial fibrillary acidic protein (GFAP).②In vitro

MSCs were cultured with modified neuronal medium(MNM)for 18 hours after being pretreated with 0.5umol/L all-trans retinoid acid (ATRA) for 24 hours.Immunocytochemistry was used to detect the expression of nestin

MAP-2 and GFAP.Results:①After being injected into rats for 2 weeks

MSCs survived in the ischemic areas and a few cells expressed nestin

MAP-2 and GFAP respectively.②In vitro

after ATRA and MNM treatment

the positive percentage of nestin and MAP-2 were (92.3±3.4)% and (89.6±3.3)% respectively

but no expression of GFAP was detected.Conclusion:The different ratio of MSCs differentiating into neural cells suggests that there is difference in the process and mechanism between environment in vitro and in vivo.

关键词

骨髓间充质干细胞神经细胞分化

Keywords

mesenchymal stem cellsNeural cellsdifferentiation

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