RNA interference c-erbB-2 and c-raf-1 genes expression combined transfection in the Tca8113 cell lines

JI Ping; et al

doi:10.13406/j.cnki.cyxb.2004.05.003

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RNA interference c-erbB-2 and c-raf-1 genes expression combined transfection in the Tca8113 cell lines

更新时间：2024-01-17

- RNA interference c-erbB-2 and c-raf-1 genes expression combined transfection in the Tca8113 cell lines
- Journal of Chongqing Medical University Issue 5, Pages: 567-571(2004)
- 作者机构：
  
  1. 重庆医科大学临床学院颌面外科
  2. 重庆医科大学临床学院颌面外科,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2004.05.003
  CLC： R739.8
- Published：2004，
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[1]季平,李庆妹,张福军,李少林.RNAi沉默c-erbB-2、c-raf-1表达对人舌鳞癌Tca8113细胞的干预作用[J].重庆医科大学学报,2004(05):567-571.

JI Ping, et al. RNA interference c-erbB-2 and c-raf-1 genes expression combined transfection in the Tca8113 cell lines. [J]. Journal of Chongqing Medical University (5):567-571(2004)
[1]季平,李庆妹,张福军,李少林.RNAi沉默c-erbB-2、c-raf-1表达对人舌鳞癌Tca8113细胞的干预作用[J].重庆医科大学学报,2004(05):567-571. DOI： 10.13406/j.cnki.cyxb.2004.05.003.

JI Ping, et al. RNA interference c-erbB-2 and c-raf-1 genes expression combined transfection in the Tca8113 cell lines. [J]. Journal of Chongqing Medical University (5):567-571(2004) DOI： 10.13406/j.cnki.cyxb.2004.05.003.

摘要

目的 :探讨应用RNA干扰技术沉默c-erbB - 2、c -raf- 1基因表达

研究其对人舌鳞癌Tca8113细胞增殖的抑制作用及其分子机制。方法 :实验分成 3组 :空白对照组、正常对照组、c -erbB - 2、c-raf- 1dsRNA联合干扰组。脂质体转染后不同时间分别进行MTT试验、电镜检测、RT -PCR

以观察不同条件处理后各组细胞的增殖、凋亡、细胞周期、mRNA及蛋白质表达有无差别。结果 :c-erbB - 2、c -raf- 1dsRNA联合干扰组

转染 72h的OD值为 0 .0 73（P <0 .0 0 5 ）

增殖抑制率达到了94 .5 %

凋亡率为 76 .5 % （P <0 .0 1）

明显地下调c-erbB - 2与c -raf- 1基因mRNA及其蛋白质的表达水平。结论 :在舌癌Tca8113细胞株增殖的抑制作用研究中

c-erbB - 2、c -raf- 1dsRNA联合干扰具有显著沉默c -erbB - 2、c -raf- 1基因表达。

Abstract

Objective:To investigate the proliferating inhibition and the mechanisms of dsRNA interference c-erbB-2 and c-raf-1 genes expression combined transfection in the human tongue carcinoma Tca8113 cell lines.Methods:There were 3 groups in our study

control group

normal control group and RNAi experimental group.At different time after liposome-mediated transfection

the cell proliferation

apoptosis

mRNA level

protein expressing level and cell cycle were observed by MTT

RT-PCR and electronic microscope.Results:According to the results of RNAi experimental group

the OD-value were 0.073( P <0.005)transfected after 72h;the inhibitory rate of proliferation were 94.5%;the apoptosis rate were 76.5% ( P <0.01 ) and the level of mRNA and protein were decreased statistically in both c-erbB-2 and c-raf-1.Conclusion:These results suggest that c-erbB-2 and c-raf-1 dsRNA effects of silencing c-erbB-2 and c-raf-1 genes expression combined transfection of tongue carcinoma.

关键词

舌鳞癌RNA干扰转录后沉默（PTGS）联合转染

Keywords

Tongue carcinomaRNA interferingPost transcriptional gene silencing(PTGS)Combined transfection

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