Application and evaluation of BECKMAN CX<sub>3</sub> DELTA in determing total protein in urine and cerebrospinal fluid

ZENG Zhulun; et al

doi:10.13406/j.cnki.cyxb.2004.04.045

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Application and evaluation of BECKMAN CX₃ DELTA in determing total protein in urine and cerebrospinal fluid

更新时间：2024-01-17

- Application and evaluation of BECKMAN CX₃ DELTA in determing total protein in urine and cerebrospinal fluid
- Journal of Chongqing Medical University Issue 4, Pages: 540-543(2004)
- 作者机构：
  
  1. 重庆医科大学第二临床学院检验科
  2. 四川省人民医院检验科,重庆,400010
  3. ,成都,610000
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2004.04.045
  CLC： R446.1
- Published：2004，
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[1]曾祝伦,张莲.BECK MAN CX_3 DELTA检测脑脊液和尿液总蛋白的应用与评价[J].重庆医科大学学报,2004(04):540-543.

ZENG Zhulun, et al. Application and evaluation of BECKMAN CX₃ DELTA in determing total protein in urine and cerebrospinal fluid. [J]. Journal of Chongqing Medical University (4):540-543(2004)
[1]曾祝伦,张莲.BECK MAN CX_3 DELTA检测脑脊液和尿液总蛋白的应用与评价[J].重庆医科大学学报,2004(04):540-543. DOI： 10.13406/j.cnki.cyxb.2004.04.045.

ZENG Zhulun, et al. Application and evaluation of BECKMAN CX₃ DELTA in determing total protein in urine and cerebrospinal fluid. [J]. Journal of Chongqing Medical University (4):540-543(2004) DOI： 10.13406/j.cnki.cyxb.2004.04.045.

摘要

目的 :评价BECKMANCX3 DELTA测定脑脊液和尿液总蛋白的优缺点。方法 :BECKMANCX3 DELTA（以下简称CX3 ）改良双缩脲法测定脑脊液和尿液总蛋白

与磺基水杨酸法相比较。结果 :CX3 改良双缩脲法和磺基水杨酸法测定脑脊液总蛋白结果（ x±s）分别为 30 3.85± 80 .4 4mg/L和 2 97.3± 79.4 3mg/L

两法测定结果无显著性差异（P >0 .0 5 ） ;尿液标本无论是正常对照组或病人组

两法的测定结果均有显著性差异（P <0 .0 1）。其中维生素C、尿酸对尿蛋白测定有正干扰 ;加入30 %H2 O2 可消除一部分尿酸等还原物质的干扰

但不甚理想。结论 :BECKMANCX3 DELTA能快速准确地测定脑脊液蛋白

但由于尿液成份复杂

在测定尿蛋白时存在较大偏差

不适合临床采用

Abstract

Objective:To evaluate the determination of total protein in cerebrospinal fluid and urine using BECKMAN CX

DELTA.Methods:The improved biuret method of BECKMAN CX

DELTA in determining total protein in urine and cerebrospinal fluid

was compared with sulfosalicylic acid method in OLYMPUS AU-560.Results:The result of total protein in cerebrospinal fluid determined with these two methods was （±s）303.85±80.44mg/L and 297.31±79.43mg/L respectively

showing no significant difference between them（P

0.05）.As to the urine whether it is from the normal group or the patient group

there was always significant difference between two methods（P

0.01）

and vitamin C and uric acid both added positive distraction to the results.The addition of 30% H

could help to clear off the distraction

but not satisfactorily.Conclusion:BECKMAN CX

DELTA can determine the total protein in cerebrospinal fluid quickly and accurately.While as urine is complex

errors always occur in the determination

it is not suitable.

关键词

尿液蛋白脑脊液蛋白双缩脲磺基水杨酸

Keywords

Urinary proteinProtein in cerebrospinal fluidBiuret reactionSulfosalicylic acid

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Application and evaluation of BECKMAN CX3 DELTA in determing total protein in urine and cerebrospinal fluid

DOI：10.13406/j.cnki.cyxb.2004.04.045

摘要

Abstract

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Application and evaluation of BECKMAN CX₃ DELTA in determing total protein in urine and cerebrospinal fluid