Growth-inhibiting effects of oxaliplatin on K562 cells in vitro

CHEN Shu; et al

doi:10.13406/j.cnki.cyxb.2004.04.013

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Growth-inhibiting effects of oxaliplatin on K562 cells in vitro

更新时间：2024-01-17

- Growth-inhibiting effects of oxaliplatin on K562 cells in vitro
- Journal of Chongqing Medical University Issue 4, Pages: 452-454(2004)
- 作者机构：
  
  1. 重庆医科大学第二临床学院血液科
  2. 重庆医科大学病理生理教研室
  3. 重庆医科大学第二临床学院血液科,重庆,400010
  4. ,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2004.04.013
  CLC： R733.7
- Published：2004，
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[1]陈姝,汤为学,娄世锋.奥沙利铂对K562细胞体外生长的影响[J].重庆医科大学学报,2004(04):452-454+458.

CHEN Shu, et al. Growth-inhibiting effects of oxaliplatin on K562 cells in vitro. [J]. Journal of Chongqing Medical University (4):452-454(2004)
[1]陈姝,汤为学,娄世锋.奥沙利铂对K562细胞体外生长的影响[J].重庆医科大学学报,2004(04):452-454+458. DOI： 10.13406/j.cnki.cyxb.2004.04.013.

CHEN Shu, et al. Growth-inhibiting effects of oxaliplatin on K562 cells in vitro. [J]. Journal of Chongqing Medical University (4):452-454(2004) DOI： 10.13406/j.cnki.cyxb.2004.04.013.

摘要

目的 :观察奥沙利铂对人白血病K5 6 2细胞的作用。方法 :检测奥沙利铂对K5 6 2细胞作用的时间效应和剂量效应

在电镜下观察奥沙利铂作用后细胞形态变化 ;用流式细胞仪分析细胞周期的改变。结果 :随着作用时间及药物浓度的增加

奥沙利铂对K5 6 2细胞的增殖抑制作用就越明显。 0 .0 16mmol/L奥沙利铂作用 72h

K5 6 2细胞生长抑制率达 85 %

形态学及流式细胞仪分析显示G2 /M期阻滞和细胞凋亡。结论 :奥沙利铂诱发的人白血病细胞的凋亡与细胞分裂期阻滞密切相关

Abstract

Objective:To investigate the effects of oxaliplatin on human leukemia K562 cell and its mechanism.Methods:The leukemia cell growth was measured by MTT-microculture tetrazolium assay.Cell-cycle kinetics and apoptosis were analyzed by flow cytometry and microscopic examination.Results:Oxaliplatin inhibited the leukemia cell growth in concentration- and time-dependent manners.Marked cells accumulated in G

/M phase.The rate of cell growth inhibition was 85% after intervention in 0.016 mmol/L oxaliplatin for 72 hours.The cells showed G

/M arrest and apoptosis.Conclusion:Oxaliplatin suppresses the growth of K562 cells in vitro by causing cell-cycle arrest

and cell apoptosis.The mechanisms of oxaliplatin provide theoretical guidance for clinical treatment.

关键词

奥沙利铂白血病细胞凋亡

Keywords

oxaliplatinleukemiaapoptosis

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