Experimental study on angiotensin Ⅱ and podocyte injury in diabetic rats

QIN Qiaojing; et al

doi:10.13406/j.cnki.cyxb.2004.04.012

您当前的位置：

首页 >

文章列表页 >

Experimental study on angiotensin Ⅱ and podocyte injury in diabetic rats

更新时间：2024-01-17

- Experimental study on angiotensin Ⅱ and podocyte injury in diabetic rats
- Journal of Chongqing Medical University Issue 4, Pages: 448-451(2004)
- 作者机构：
  
  1. 重庆医科大学临床学院内分泌科
  2. 重庆医科大学临床学院内分泌科,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2004.04.012
  CLC： R587.1
- Published：2004，
扫描看全文
[1]覃乔静,邓华聪,曹文富,兰丽珍,刘东方.血管紧张素Ⅱ与糖尿病大鼠足细胞损害的实验研究[J].重庆医科大学学报,2004(04):448-451+502.

QIN Qiaojing, et al. Experimental study on angiotensin Ⅱ and podocyte injury in diabetic rats. [J]. Journal of Chongqing Medical University (4):448-451(2004)
[1]覃乔静,邓华聪,曹文富,兰丽珍,刘东方.血管紧张素Ⅱ与糖尿病大鼠足细胞损害的实验研究[J].重庆医科大学学报,2004(04):448-451+502. DOI： 10.13406/j.cnki.cyxb.2004.04.012.

QIN Qiaojing, et al. Experimental study on angiotensin Ⅱ and podocyte injury in diabetic rats. [J]. Journal of Chongqing Medical University (4):448-451(2004) DOI： 10.13406/j.cnki.cyxb.2004.04.012.

摘要

目的 :研究血管紧张素Ⅱ （AngⅡ ）在糖尿病大鼠肾小球滤过屏障外层足细胞损害中的作用

以探讨糖尿病肾病的发生机制。方法 :实验大鼠分为 3组 :正常对照组（C）、糖尿病组（D）和氯沙坦治疗病鼠组（DT）

实验第 5周末检测肾皮质超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、丙二醛（MDA）和尿白蛋白排泄率（UAER）的水平

利用透射电子显微镜观察肾脏足细胞超微结构

同时用免疫组化技术检测肾小球足细胞损伤标志蛋白 -desmin蛋白的表达。结果 :与正常对照组比较

糖尿病组大鼠肾皮质SOD和CAT活性显著降低

而MDA含量明显增加

肾小球内desmin蛋白表达上调

足细胞部分足突融合

尿白蛋白排泄率增高 ;AngⅡ 1型受体拮抗剂氯沙坦明显减轻糖尿病大鼠肾皮质SOD、CAT、MDA及肾小球内desmin蛋白表达的改变

减轻足细胞足突融合

降低尿白蛋白排泄率。结论 :糖尿病时AngⅡ参与增高肾组织氧化应激

损害肾小球滤过屏障外层足细胞的结构和功能

促使糖尿病肾脏损害和蛋白尿的发生

Abstract

Objective:To investigate the role of angiotensin Ⅱ(Ang Ⅱ) in the injury of podocytes

last barrier of glomemlar filtration

to research the pathogenesis of diabetic nephropathy.Methods:The experimental rats were divided into three groups:control rats(group C)

diabetic rats(group D)and diabetic rats treated by losartan(group DT).Urinary albumin excretion rate (UAER) was assayed 5 weeks after treatment.In addition

the activity of superoxide dismutase(SOD)

catalase (CAT) and concentration of matondialdehyde(MDA) in renal cortex were measured.The podocyte ultrastructure was observed by transmission electronic microscopy while immunohistochemistry was used to detect the expression of desmin

a marker of podocyte injury.Results:Compared with control rats

the activity of SOD and CAT was decreased significantly and the concentrations of MDA were increased markedly in diabetic renal cortex.The expression of glomerular desmin increased markedly accompanied with foot process effacement in podocytes in diabetic group.At the same time

UAER increased in diabetes.The changes of SOD

CAT

MDA

glomemlar desmin expression

UAER and foot process effacement were markedly ameliorated after diabetic rats were treated by angiotensin Ⅱ type one receptor antagonist losartan.Conclusion:Ang Ⅱ may participate in the structural and functional damage of podocytes

last barrier of glomerular filtration

by increasing renal oxidative stress to injure kidney and accelerate proteinuria in diabetes.

关键词

糖尿病肾病血管紧张素Ⅱ氧化应激足细胞

Keywords

diabetic nephropathyAngiotensin Ⅱoxidative stressPodocyte

references

Views

276

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Carvacrol alleviates oxidative stress damage in rats with diabetic nephropathy via the SIRT1/FOXO1 pathway

Relationship of gene expression and activity of chymase and Ang Ⅱ,nephropathy in streptozotocin-induced diabetic hamsters

Liver pathophysiology of Sprague-Dawley rat models of intrahepatic cholestasis of pregnancy induced by different sex hormones

Research progress of molecular mechanism of palmitic acid-induced pancreatic β-cell dysfunction

Protective effect of sodium aescinate against hydronephrosis injury in mice after unilateral ureteral obstruction

Related Author

Liu Xia

Wang Xia

Yang Bingqi

Liu Xia

CHENG Li

et al

蒲霞

李龙英

Related Institution

Department of Obstetrics and Gynecology，Hebei Provincial Hospital of Traditional Chinese Medicine

河北省中医院导管室

Department of Geriatrics, the First Affiliated Hospital, Chongqing Medical University

四川省成都市金牛区成都铁路中心医院内分泌科

Department of Obstetrics and Gynecology，The First Affiliated Hospital，Institute of Life Science， Chongqing Medical University

⁰