Study on arsenic trioxide induced apoptosis in hepatoma cells andits molecular mechanism

ZHANG Xia; et al

doi:10.13406/j.cnki.cyxb.2004.04.011

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Study on arsenic trioxide induced apoptosis in hepatoma cells andits molecular mechanism

更新时间：2024-01-17

- Study on arsenic trioxide induced apoptosis in hepatoma cells andits molecular mechanism
- Journal of Chongqing Medical University Issue 4, Pages: 444-447(2004)
- 作者机构：
  
  1. 重庆医科大学临床学院消化内科
  2. 重庆医科大学临床学院消化内科,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2004.04.011
  CLC： R735.7
- Published：2004，
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[1]张霞,王丕龙,陶小红.三氧化二砷诱导人肝癌细胞凋亡及相关分子机制研究[J].重庆医科大学学报,2004(04):444-447.

ZHANG Xia, et al. Study on arsenic trioxide induced apoptosis in hepatoma cells andits molecular mechanism. [J]. Journal of Chongqing Medical University (4):444-447(2004)
[1]张霞,王丕龙,陶小红.三氧化二砷诱导人肝癌细胞凋亡及相关分子机制研究[J].重庆医科大学学报,2004(04):444-447. DOI： 10.13406/j.cnki.cyxb.2004.04.011.

ZHANG Xia, et al. Study on arsenic trioxide induced apoptosis in hepatoma cells andits molecular mechanism. [J]. Journal of Chongqing Medical University (4):444-447(2004) DOI： 10.13406/j.cnki.cyxb.2004.04.011.

摘要

目的 :研究三氧化二砷（As2 O3 ）诱导人肝癌细胞凋亡时

细胞周期及细胞周期调节蛋白的变化

探讨As2 O3 抗肝癌作用的分子机制。方法 :体外培养人肝癌细胞株SMMC - 772 1

用 2 μmol/LAs2 O3 处理 72h

流式细胞仪检测细胞周期变化及细胞凋亡率 ;透射电镜观察细胞形态变化及细胞凋亡情况 ;免疫细胞化学检测cyclinD1、cyclinA、p2 1蛋白的表达。结果 :As2 O3使SMMC - 772 1细胞周期阻滞在G0 /G1、S期

与对照组比较

As2 O3 在诱导SMMC - 772 1细胞发生凋亡的同时

p2 1蛋白的表达水平显著增高（对照组 1.12 8;As2 O3 组 :3.794 ）

cyclinD1、cyclinA蛋白的表达水平明显下降（对照组 :3.6 4 7

2 .833;As2 O3组 :1.133

1.179）。结论 :As2 O3 能干扰细胞周期的进程

诱导SMMC - 772 1细胞凋亡。其作用机制与上调p2 1蛋白及下调cyclinD1、cyclinA蛋白的表达有关

Abstract

Objective:To study the changes of cell cycle phase and cell cycle phase regulation protein during arsenic trioxide induced apoptosis in hepatoma cells

and to explore mechanisms of arsenic trioxide inhibited HCC.Methods:Cultured in vitre

HCC SMMC-7721 were treated 72h with 2μmol/L arsenic trioxide.The cell cycle and apoptosis index were detected by flow cytometry（FCM）;the morphological changes were observed by transmission electron microscope.The immunohistochemistry was used to detect the protein expression of p21、cyclin D

cyclinA.Results:SMMC-7721 cell cycle was arrested in G

and S phase by arsenic trioxide.Compared with control in the course of apoptosis induced by arsenic trioxide p21 protein expressions rose significantly（control:1.128;arsenic trioxide:3.794）

while cyclinD

cyclinA protein expressions decreased significantly（control 1:3.647

2.833;arsenic trioxide:1.133

1.179）.Conclusion:Arsenic trioxide could disturb cell cycle progression of SMMC-7721 and induce apoptosis.The mechanism may relate to that arsenic trioxide up-regulate expression of p21 and down-regulate expression of cyclinD

cyclinA.

关键词

三氧化二砷肝细胞癌凋亡细胞周期调节蛋白

Keywords

arsenic trioxideHepatomaapoptosiscell cycleRegulation protein

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