Cervical tumor lysate-loaded dendritic cell induce the generation of lymphcyte

JIA Ying; et al

doi:10.13406/j.cnki.cyxb.2004.04.005

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Cervical tumor lysate-loaded dendritic cell induce the generation of lymphcyte

更新时间：2024-01-17

- Cervical tumor lysate-loaded dendritic cell induce the generation of lymphcyte
- Journal of Chongqing Medical University Issue 4, Pages: 421-424(2004)
- 作者机构：
  
  1. 重庆医科大学临床学院妇产科
  2. 重庆医科大学临床学院妇产科,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2004.04.005
  CLC： R737.33
- Published：2004，
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[1]贾英,黄裕,唐良萏.负载宫颈癌肿瘤裂解物的树突状细胞诱导的淋巴细胞反应[J].重庆医科大学学报,2004(04):421-424.

JIA Ying, et al. Cervical tumor lysate-loaded dendritic cell induce the generation of lymphcyte. [J]. Journal of Chongqing Medical University (4):421-424(2004)
[1]贾英,黄裕,唐良萏.负载宫颈癌肿瘤裂解物的树突状细胞诱导的淋巴细胞反应[J].重庆医科大学学报,2004(04):421-424. DOI： 10.13406/j.cnki.cyxb.2004.04.005.

JIA Ying, et al. Cervical tumor lysate-loaded dendritic cell induce the generation of lymphcyte. [J]. Journal of Chongqing Medical University (4):421-424(2004) DOI： 10.13406/j.cnki.cyxb.2004.04.005.

摘要

目的 :利用树突状细胞（dendriticcell

DC）递呈肿瘤抗原的生物学特性

观察DC负载宫颈肿瘤裂解物抗原后能否促进同种异体T淋巴细胞增殖

并且活化为细胞毒性T淋巴细胞（cytotoxicTlymphocyte

CTL）。方法 :宫颈癌患者外周血单核细胞体外经粒 -巨噬细胞集落刺激因子（GM -CSF）和白细胞介素 - 4 （IL - 4 ）诱导产生DC

肿瘤裂解物和TNF -α共同促其成熟

与异体T淋巴细胞混合共育

以MTT法测定T细胞的增殖程度。结果 :宫颈癌患者外周血来源DC无论负载肿瘤组织裂解物还是传代肿瘤细胞裂解物

均可促进异体T细胞大量增殖

二者间无显著差异（P <0 .0 5 ）。结论 :DC能递呈宫颈癌肿瘤抗原

诱导产生大量抗原特异性CTL

提示负载肿瘤裂解物的DC具有为宫颈癌患者进行特异性免疫治疗的临床应用前景

Abstract

Objective:To make use of the characteristics of presenting and processing tumor antigen of dendritic cell to enhance generation of isotypical but xenogenous T lymphocyte.Methods:Autologous dendritic cells were obtained by culturing peripheral blood monocyte of patients with cervical cancer in the presence of granulocyte-macrophage colony stimulating factor(GM-CSF) and interleukin-4(IL-4).Dendritic cells were loaded with tumor cell lysate and co-cultured with heterogenous PBMCs from patients to induce generation of tumor specific cytotoxic T cell.The generation rate was measured by MTT.Results:Tumor lysate-loaded DC which was isolated from peripheral blood monocyte of patients with cervical cancer could induce the generation of isotypical but xenogenous T lymphcyte.There was no significant difference between tumor tissue lysate and tumor cell strain lysate(P<0.05).Conclusion:These results indicate that DC could process and present tumor lysate antigen to stimulate generation of specific immunotherapy in patients with cervical cancer.

关键词

宫颈肿瘤树突状细胞肿瘤裂解物T淋巴细胞

Keywords

Cervical carcinomadendritic cellTumor lysateT lymphcyte

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