Effect of retroviral HSVtk gene transfer on mice bladder tumor growth in vivo

YE Gang; et al

doi:10.13406/j.cnki.cyxb.2004.04.004

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Effect of retroviral HSVtk gene transfer on mice bladder tumor growth in vivo

更新时间：2024-01-17

- Effect of retroviral HSVtk gene transfer on mice bladder tumor growth in vivo
- Journal of Chongqing Medical University Issue 4, Pages: 417-420(2004)
- 作者机构：
  
  1. 第三军医大学新桥医院泌尿外科
  2. 第三军医大学新桥医院泌尿外科,重庆,400037
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2004.04.004
  CLC： R737.14
- Published：2004，
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[1]叶钢,刘为池,张荣贵.体内转导逆转录病毒HSVtk基因对鼠膀胱癌生长的影响[J].重庆医科大学学报,2004(04):417-420.

YE Gang, et al. Effect of retroviral HSVtk gene transfer on mice bladder tumor growth in vivo. [J]. Journal of Chongqing Medical University (4):417-420(2004)
[1]叶钢,刘为池,张荣贵.体内转导逆转录病毒HSVtk基因对鼠膀胱癌生长的影响[J].重庆医科大学学报,2004(04):417-420. DOI： 10.13406/j.cnki.cyxb.2004.04.004.

YE Gang, et al. Effect of retroviral HSVtk gene transfer on mice bladder tumor growth in vivo. [J]. Journal of Chongqing Medical University (4):417-420(2004) DOI： 10.13406/j.cnki.cyxb.2004.04.004.

摘要

目的 :观测瘤体和腹腔内注射逆转录病毒载体HSVtk基因对膀胱癌的作用。方法 :用鼠膀胱癌细胞（T739）分别建立鼠背部和腹腔肿瘤模型。含HSVtk基因的病毒上清瘤体和腹腔内注射

联合丙氧鸟苷（ganciclovir

GCV）治疗观察肿瘤大小及动物存活时间。结果 :接种后约 1周所有动物长出肿瘤。HSVtk/GCV处理后

组织原位杂交显示瘤细胞内HSVtk基因mRNA表达。背部和腹腔肿瘤生长明显受到抑制

动物平均存活时间延长（P <0 .0 5 ）结论 :瘤体和腹腔内注射逆转录病毒载体能将HSVtk基因导入膀胱癌细胞

联合GCV治疗显示出较强的抑瘤效应

Abstract

Objective:To evaluate the effectiveness of intratumoral and intraperitoneal injection with retrovirus HSVtk gene on mouse bladder cancer.Methods:Subcutaneous and intraperitoneal bladder tumor was respectively established with mouse cancer cell line(T739) in syngeneic mice.Direct intratumor injection of retroviral HSVtk into the subcutaneous tumor was performed.In peritoneum cavity tumor model

HSVtk gene was transferred by intraperitoneal injection of retrovirus vector carrying HSVtk gene.After GCV therapy the tumor volume and animal survival time were monitored.Results:Both animal models were successfully induced in all mice within one week following subcutaneous and intraperitoneal injection of T739 cells.Compared with control animals

the tumor growth in both models was significantly inhibited with HSVtk/GCV system

and also the animal survival time was prolonged with administration of GCV(P<0.05).Tissue hybridization in situ confirmed the expression of HSVtk gene in mouse bladder tumors.Conclusion:Bladder tumor inoculated in mice can be retrovirally transfected with HSVtk gene by intra-tumor or intra peritoneal injection.Combined with administration of GCV

HSVtk gene significantly inhibits the bladder tumor growth.

关键词

膀胱癌逆转录病毒HSVtk丙氧鸟苷基因治疗

Keywords

bladder cancerRetrovirusHSVtkGCVgene therapy

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