Over-expression and purification of recombinant spike protein fragment of SARS coronavirus

PU Dan; et al

doi:10.13406/j.cnki.cyxb.2004.04.002

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Over-expression and purification of recombinant spike protein fragment of SARS coronavirus

更新时间：2024-01-17

- Over-expression and purification of recombinant spike protein fragment of SARS coronavirus
- Journal of Chongqing Medical University Issue 4, Pages: 409-412(2004)
- 作者机构：
  
  1. 重庆医科大学病毒性肝炎研究所
  2. 重庆医科大学病毒性肝炎研究所,重庆,400010
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2004.04.002
  CLC： R346
- Published：2004，
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[1]蒲丹,宋文鑫,闫歌,张秉强,唐霓,王丕龙,郑建,黄爱龙.重组SARS-CoV刺突蛋白基因片段的原核表达及纯化[J].重庆医科大学学报,2004(04):409-412.

PU Dan, et al. Over-expression and purification of recombinant spike protein fragment of SARS coronavirus. [J]. Journal of Chongqing Medical University (4):409-412(2004)
[1]蒲丹,宋文鑫,闫歌,张秉强,唐霓,王丕龙,郑建,黄爱龙.重组SARS-CoV刺突蛋白基因片段的原核表达及纯化[J].重庆医科大学学报,2004(04):409-412. DOI： 10.13406/j.cnki.cyxb.2004.04.002.

PU Dan, et al. Over-expression and purification of recombinant spike protein fragment of SARS coronavirus. [J]. Journal of Chongqing Medical University (4):409-412(2004) DOI： 10.13406/j.cnki.cyxb.2004.04.002.

摘要

目的 :冠状病毒（SARS -CoV）的刺突蛋白（spikeglycoprotein

S蛋白）是病毒的主要结构蛋白之一

也是重要的病毒抗原

重组S蛋白的表达可用于检测病毒感染后血清抗体。方法 :根据抗原性预测分析结果

将S蛋白中抗原决定簇的编码基因重新拼接成两个新的基因

通过全基因合成方式直接合成至原核表达载体pET32a（+）

转化大肠杆菌BL2 1（DE3）

以IPTG诱导表达

Ni-NTA试剂盒纯化目的蛋白。结果 :SDS -PAGE证实重组S蛋白的表达

并被纯化。结论 :重组刺突蛋白的表达及纯化

可做为检测SARS -CoV抗体的抗原

有助于进一步开展SARS -CoV相关研究

Abstract

Objective:To obtain the spike glycoprotein(S protein) of SARS coronavirus (SARS-Cov) for detection of the corresponding antibody and preparation of diagnostic kits.Methods:Genes encoding S protein were picked out to form a new gene and divided into two parts according to the prediction of antigenicty.They were obtained by nucleotide synthesis and were cloned into expression vector pET32a(+) respectively.Proteins then were induced by IPTG and purified through Ni-NTA.Results:Target proteins were successfully expressed and purified.Conclusion:The recombinant S proteins of SARS-CoV could be of great help in diagnosing the disease and investigating the virus further.

关键词

SARS-CoV刺突蛋白表达纯化

Keywords

SARS coronavirusSpike proteinexpressionpurification

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Related Institution

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