Establishment and MDR characteristics of HepG2/Adm nude mice xenograft

YANG Zhihong; et al

doi:10.13406/j.cnki.cyxb.2004.04.001

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Establishment and MDR characteristics of HepG2/Adm nude mice xenograft

更新时间：2024-01-17

- Establishment and MDR characteristics of HepG2/Adm nude mice xenograft
- Journal of Chongqing Medical University Issue 4, Pages: 405-408(2004)
- 作者机构：
  
  1. 重庆医科大学医学超声工程研究所
  2. 重庆医科大学医学超声工程研究所,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2004.04.001
  CLC： R735.7
- Published：2004，
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[1]杨志宏,翟宝进,邵泽勇,伍烽,王智彪.HepG2/Adm裸鼠移植瘤的建立及多药耐药特性评价[J].重庆医科大学学报,2004(04):405-408.

YANG Zhihong, et al. Establishment and MDR characteristics of HepG2/Adm nude mice xenograft. [J]. Journal of Chongqing Medical University (4):405-408(2004)
[1]杨志宏,翟宝进,邵泽勇,伍烽,王智彪.HepG2/Adm裸鼠移植瘤的建立及多药耐药特性评价[J].重庆医科大学学报,2004(04):405-408. DOI： 10.13406/j.cnki.cyxb.2004.04.001.

YANG Zhihong, et al. Establishment and MDR characteristics of HepG2/Adm nude mice xenograft. [J]. Journal of Chongqing Medical University (4):405-408(2004) DOI： 10.13406/j.cnki.cyxb.2004.04.001.

摘要

目的 :建立一种人肿瘤多药耐药（MDR）细胞株裸鼠移植瘤模型并探讨其多药耐药机制

为体内研究逆转肿瘤多药耐药提供模型。方法 :SPF级动物常规饲养

裸鼠腋窝皮下接种 1× 10 9个细胞

观察其成瘤率及生长特性 ;MTT法检测药物敏感性 ;流式细胞术、PR -PCR法检测裸鼠移植瘤细胞与原代细胞的耐药特征。结果 :HepG2 /Adm裸鼠移植瘤的成瘤率10 0 % ;HepG2 /Adm细胞及HepG2 /Adm裸鼠移植瘤对阿霉素（ADM ）的IC50 分别为 4 .2 1μg/ml和 4 .10 μg/ml

两者比较未见显著性差异（P >0 .0 5 ）。原代及裸鼠体内HepG2 /Adm的P - gp、MRP及LRP的表达率分别为 92 %和 91.8%

88.7%和 88.1%、81.1%和 80 .7%

差异无显著意义 ;二者MDR相关基因mRNA表达量无差异（P >0 .0 1）。结论 :以细胞建立的裸鼠移植瘤模型具有多药耐药特性

为研究人肿瘤MDR提供了理想的动物模型

Abstract

Objective:To establish an animal model of HepG2/Adm nude mice xenograft and investigate the mechanism of multidrug resistance（MDR）.Methods:HepG2/Adm cells were transplanted into the subcutaneous tissue in athymic nude mice.The differences in the MDR characteristics between HepG2/Adm and nude mice xenografts（HepG2/Adm/nude）were investigated.Cytotoxicity was determined with MTT assay.The expression of MDR-related gene protein and mRNA was examined by flow cytometry（FCM）and reverse transcriptase polymerase chain reaction （RT-PCR） respectively.Results:Tumor incidence was 100% after subcutaneous implantation with 10

of cells.IC

values （the concentration producing 50% maximal inhibition） of Adriamycin（ADM） to HepG2/Adm and HepG2/Adm/nude were 4.21 μg/ml

4.10μg/ml

respectively（P

0.05）.The expressions of MDR protein （P-gp

MRP and LRP）in HepG2/Adm and HepG2/Adm/nude were 92% and 91.8%

88.7% and 88.1%

81.1% and 80.7%

respectively（P

0.05）.The expression of MDR-related gene mRNA in HepG2/Adm/nude had no significant differences from that of HepG2/Adm（MDR）（P

0.05）.Conclusion:HepG2/Adm/nude xenograft established by HepG2/Adm cells transplantation retains the characteristics of MDR phenotype.It can provide an ideal animal model for the study of human tumor MDR.

关键词

肿瘤多药耐药裸鼠移植模型

Keywords

TumourMultidrug resistance(MDR)nude miceImplantationmodel

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重庆医科大学基础医学院药理教研室重庆医科大学基础医学院药理教研室硕士生

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