Aspirin induces apoptosis on human myeloid leukemia cell line HL-60

LUO Yun; et al

doi:10.13406/j.cnki.cyxb.2004.03.020

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Aspirin induces apoptosis on human myeloid leukemia cell line HL-60

更新时间：2024-01-17

- Aspirin induces apoptosis on human myeloid leukemia cell line HL-60
- Journal of Chongqing Medical University Issue 3, Pages: 318-321(2004)
- 作者机构：
  
  1. 重庆医科大学第二临床学院血液科
  2. 重庆医科大学临床学院血液科,重庆,400010
  3. ,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2004.03.020
  CLC： R733.7
- Published：2004，
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[1]罗云,唐宗山,黄宗干.阿司匹林诱导HL-60细胞凋亡的实验研究[J].重庆医科大学学报,2004(03):318-321.

LUO Yun, et al. Aspirin induces apoptosis on human myeloid leukemia cell line HL-60. [J]. Journal of Chongqing Medical University (3):318-321(2004)
[1]罗云,唐宗山,黄宗干.阿司匹林诱导HL-60细胞凋亡的实验研究[J].重庆医科大学学报,2004(03):318-321. DOI： 10.13406/j.cnki.cyxb.2004.03.020.

LUO Yun, et al. Aspirin induces apoptosis on human myeloid leukemia cell line HL-60. [J]. Journal of Chongqing Medical University (3):318-321(2004) DOI： 10.13406/j.cnki.cyxb.2004.03.020.

摘要

目的 :研究非甾体类抗炎药阿司匹林（ASA）对体外诱导人急性白血病细胞的凋亡作用及其相关机制。方法 :采用MTT法测定ASA对HL - 6 0细胞的抑制率

通过光镜、流式细胞仪（FCM）、原位末端标记法（TUNEL）观测ASA诱导HL - 6 0细胞凋亡

半定量RT -PCR检测基因表达。结果 :浓度为 2 .5～ 10mmol/L的ASA能抑制HL - 6 0细胞增殖

诱导细胞凋亡 ;ASA与阿糖胞苷（Ara -c）抗肿瘤作用具有相加效应 ;ASA可下调HL - 6 0细胞c -mycmRNA水平表达。结论 :一定浓度ASA在体外有抑制急性白血病细胞生长和诱导细胞凋亡的作用

其机制可能与c-myc基因下调有关。

Abstract

Objective:To explore the effect of aspirin(ASA) on the apoptosis of human myeloid leukemia cell in vitro and its mechanisms.Methods:Inhibition rate was tested by MTT method.Apoptosis induced by ASA in HL-60 cells was investigated by applying light microscopy

TdT-mediated dUTP Nick-end Labeling(TUNEL) and flow cytometry analysis techniques.RT-PCR method were used to examine mRNA expression.Results:2.5～10.0mmol/L ASA can induce apoptosis and inhibit proliferation in HL-60 cells

and increase the sensitivity of HL-60 cells to Ara-c.ASA down-regulated the level of c-myc mRNA.Conclusion:A certain dosage of ASA can suppress the proliferation and induce apoptosis of acute leukemia cells in a dose dependent manner.One of the mechanisms of ASA inducing apoptosis may be related to down-regulated expression of c-myc mRNA.

关键词

阿司匹林细胞凋亡HL60

Keywords

aspirinapoptosisHL-60

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