Induction of immune responses against rotavirus by intramuscular and intranasal immunization with rotavirus DNA vaccine

ZHANG Jing; et al

doi:10.13406/j.cnki.cyxb.2004.03.017

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Induction of immune responses against rotavirus by intramuscular and intranasal immunization with rotavirus DNA vaccine

更新时间：2024-01-17

- Induction of immune responses against rotavirus by intramuscular and intranasal immunization with rotavirus DNA vaccine
- Journal of Chongqing Medical University Issue 3, Pages: 308-310(2004)
- 作者机构：
  
  1. 重庆医科大学儿科学院心内科
  2. 重庆医科大学儿科研究所,重庆,400014
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2004.03.017
  CLC： R392
- Published：2004，
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[1]张静,王宁遂,邓兵,朱静.轮状病毒DNA疫苗经不同途径接种诱导体内免疫应答研究[J].重庆医科大学学报,2004(03):308-310.

ZHANG Jing, et al. Induction of immune responses against rotavirus by intramuscular and intranasal immunization with rotavirus DNA vaccine. [J]. Journal of Chongqing Medical University (3):308-310(2004)
[1]张静,王宁遂,邓兵,朱静.轮状病毒DNA疫苗经不同途径接种诱导体内免疫应答研究[J].重庆医科大学学报,2004(03):308-310. DOI： 10.13406/j.cnki.cyxb.2004.03.017.

ZHANG Jing, et al. Induction of immune responses against rotavirus by intramuscular and intranasal immunization with rotavirus DNA vaccine. [J]. Journal of Chongqing Medical University (3):308-310(2004) DOI： 10.13406/j.cnki.cyxb.2004.03.017.

摘要

目的 :通过研究不同途径接种轮状病毒DNA疫苗所诱导的体内免疫应答

寻找其适合的免疫途径。方法 :轮状病毒基因疫苗 pcDNA1/VP7经肌肉注射及鼻粘膜两种途径免疫BALB/c小鼠

利用ELISA方法对其诱导产生的体液免疫应答进行测定。结果 :经肌肉注射及鼻粘膜两种途径接种轮状病毒DNA疫苗后

均在BALB/c小鼠体内诱导产生了病毒特异性IgG增高（P <0 .0 5 ）

而病毒特异性IgA与对照组相比无显著性差异。结论 :肌肉注射及鼻粘膜两种途径均能作为轮状病毒DNA疫苗的候选接种途径

Abstract

Objective:To search for the efficient immune routes for rotavirus DNA vaccine by evaluating efficacy in inducing immune responses to rotavirus.Methods:In this study BALB/c mice were immunized by intramuscular and intranasal routes with rotavirus DNA vaccine

pcDNA 1/VP7

then rotavirus specific systemic immunity was measured.Results:Serum rotavirus specific IgG but not IgA titers of mice immunized by both routes were significantly higher than that of the unimmunized controls.Conclusion:Both intramuscular and intranasal routes are candidates for rotavirus DNA vaccine.

关键词

轮状病毒DNA疫苗粘膜免疫

Keywords

RotavirusDNA vaccineMucosal immunization

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Department of Neurology,the Children’s Hospital,Chongqing Medical University

重庆医科大学儿童医院神内科

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