Objective:To investigate the effects of 10-HCPT on the growth and proliferation of human hepatoma cell lines QGY and HepG2 in vitro.Methods:QGY and HepG2 were cultured in RPMI1640.They were treated by different concentrations of 10-HCPT for 48 hours

and by a dose of 100μg/ml of 10-HCPT for different hours.The cell growth indexes (GI) were detected by MTT colorimetric assay.The clone formation rates of QGY and HepG2 were observed by soft agar assay.Results:The GI of HepG2 and QGY cells treated by 10-HCPT with concentration 10～360μg μg/ml and 30～360μg μg/ml were significantly lower than that of control groups respectively ( P <0.05)

and the inhibitive effect increased with concentrations ranging from 30 to 360μg/ml in a dose-dependent manner.The effects of 100μg/ml10-HCPT on the growth of QGY and HepG2 cells for 24 hours were significant as compared with control groups( P< 0.05).The inhibition in 36～72 hours was highly significant ( P <0.01)

while not significant among themselves ( P >0.05).The clone formation rates of QGY and HepG2 of experimental groups were significantly lower than that of control groups respectively ( P <0.01).Conclution:10-HCPT could inhibit the growth and proliferation of human hepatoma cell lines QGY and HepG2 effectively in vitro.