The effects of curcumin on proliferation and apoptosis of human cervical carcinoma Hela cells in vitro

ZHAO Jing; et al

doi:10.13406/j.cnki.cyxb.2004.03.013

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The effects of curcumin on proliferation and apoptosis of human cervical carcinoma Hela cells in vitro

更新时间：2024-01-17

- The effects of curcumin on proliferation and apoptosis of human cervical carcinoma Hela cells in vitro
- Journal of Chongqing Medical University Issue 3, Pages: 296-298(2004)
- 作者机构：
  
  1. 重庆医科大学基础医学院病理教研室
  2. 重庆医科大学基础医学院病理教研室,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2004.03.013
  CLC： R737.33
- Published：2004，
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[1]赵敬,赵涌.姜黄素对人宫颈癌细胞株Hela细胞抑制及凋亡的影响[J].重庆医科大学学报,2004(03):296-298+314.

ZHAO Jing, et al. The effects of curcumin on proliferation and apoptosis of human cervical carcinoma Hela cells in vitro. [J]. Journal of Chongqing Medical University (3):296-298(2004)
[1]赵敬,赵涌.姜黄素对人宫颈癌细胞株Hela细胞抑制及凋亡的影响[J].重庆医科大学学报,2004(03):296-298+314. DOI： 10.13406/j.cnki.cyxb.2004.03.013.

ZHAO Jing, et al. The effects of curcumin on proliferation and apoptosis of human cervical carcinoma Hela cells in vitro. [J]. Journal of Chongqing Medical University (3):296-298(2004) DOI： 10.13406/j.cnki.cyxb.2004.03.013.

摘要

目的 :探讨姜黄素（curcumin）对人宫颈癌细胞株Hela细胞体外增殖及凋亡的调控作用。方法 :选用人宫颈癌Hela细胞进行体外培养

以 5～ 5 0 μmol/L的姜黄素分别处理Hela细胞 2 4～ 72h后

MTT法检测细胞增殖

流式细胞仪（FCM ）检测凋亡

透射电镜进行形态学观察。SABC免疫组化法检测细胞内Bcl- 2、BcI -XL、Caspase - 3蛋白表达水平。结果 :curcumin对Hela细胞增殖有抑制作用。并呈剂量依赖性。DNA直方图上可见亚“G1”峰 ;透射电镜观察见部分细胞发生凋亡形态学改变。Bcl- 2、Bcl-XL表达均下调

Caspase - 3表达增强。结论 :姜黄素对人宫颈癌Hela细胞的增殖具有明显抑制作用。上调Cas pase - 3

下调Bcl- 2、Bcl-XL蛋白的表达

诱导细胞凋亡可能是其作用机制之一。

Abstract

Objective:To investigate the regulatory effect of curcumin on proliferation and apoptosis in human cervical carcinoma cell line Hcla in vitro.Methods:Human cervical carcinoma cell line Hela was cultured in vitro.Hela cells were treated with 5-50 μmol/L curcumin for 24-72h and the growth inhibition rates of Hela cells were measured with MTT method.Cell apoptosis was inspected by electron microscopy.In addition

the expression of bcl-2

bcl-xl and caspase-3 protein in Hela cell were observed by SABC immunohistochemistry.Results:Curcumin inhibited the proliferation of Hela cells on a dose-depending manner.With the peak of sub G 1 appeared on DNA histogram in FCM.Partial cells presented the characteristic morphological changes of apoptosis under the electron microscope.The Bcl-2

Bcl-xl expression were decreased while Caspase-3 expression was increased.Conclusion:Curcumin could significantly inhibit the growth of Hela cells;inducing apoptosis through up-regulating Caspase-3 and down-regulating expression of Bcl-2

Bcl-xl is probably one of its molecular mechanisms.

关键词

姜黄素子宫颈肿瘤凋亡

Keywords

curcuminCervix neoplasmapoptosis

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