Construction and expression of the eukaryotic coexpression plasmid of Mycobacterium tuberculosis Ag85B and MPT64 gene

JIANG Shan; et al

doi:10.13406/j.cnki.cyxb.2004.03.007

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Construction and expression of the eukaryotic coexpression plasmid of Mycobacterium tuberculosis Ag85B and MPT64 gene

更新时间：2024-01-17

- Construction and expression of the eukaryotic coexpression plasmid of Mycobacterium tuberculosis Ag85B and MPT64 gene
- Journal of Chongqing Medical University Issue 3, Pages: 276-278(2004)
- 作者机构：
  
  1. 重庆医科大学微生物学及免疫学教研室
  2. 重庆医科大学微生物学及免疫学教研室,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2004.03.007
  CLC： R346
- Published：2004，
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[1]江山,朱道银,骆旭东,陈全,蒋英.结核分枝杆菌Ag85B与MPT64真核共表达质粒的构建及表达[J].重庆医科大学学报,2004(03):276-278.

JIANG Shan, et al. Construction and expression of the eukaryotic coexpression plasmid of Mycobacterium tuberculosis Ag85B and MPT64 gene. [J]. Journal of Chongqing Medical University (3):276-278(2004)
[1]江山,朱道银,骆旭东,陈全,蒋英.结核分枝杆菌Ag85B与MPT64真核共表达质粒的构建及表达[J].重庆医科大学学报,2004(03):276-278. DOI： 10.13406/j.cnki.cyxb.2004.03.007.

JIANG Shan, et al. Construction and expression of the eukaryotic coexpression plasmid of Mycobacterium tuberculosis Ag85B and MPT64 gene. [J]. Journal of Chongqing Medical University (3):276-278(2004) DOI： 10.13406/j.cnki.cyxb.2004.03.007.

摘要

目的 :构建结核分枝杆菌Ag85B和MPT6 4编码基因的共表达载体 pBud85B -MPT。方法 :将结核分枝杆菌Ag85B、MPT6 4基因同时克隆进多启动子共表达载体pBudCE4 .1中

得到真核共表达质粒 pBud85B -MPT ;将 pBud85B -MPT转染COS - 7细胞

通过RT -PCR方法检测目的基因的表达。结果 :在COS - 7细胞中同时检测到Ag85B、MPT6 4的表达。结论 :pBud85B -MPT共表达质粒构建成功

为进一步研究新型结核病疫苗奠定基础。

Abstract

Objective:To construct an eukaryotic coexpression plasmid containing Mycobacterium tuberculosis Ag85B and MPT64 gene.Methods:Mycobacterium tuberculosis Ag85B and MPT64 gene were cloned into pBudCE4.1 to construct recombinant plasmid pBud85B-MPT.The recombinant plasmid was transfected into COS-7 cells

and its expression of Ag85B and MPT64 was assesed by RT-PCR.Results:Mycobacterium tuberculosis Ag85B and MPT64 were detected in transfected COS-7 cells.Conclusion:The recombinant plasmid pBud85B-MPT can express Ag85B and MPT simultaneously in COS-7 cells which provides the basis for further investigation of DNA vaccine against tuberculosis.

关键词

结核分枝杆菌Ag85BMPT共表达载体

Keywords

mycobacterium tuberculosisAg85BMPTCoexpression vector

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Related Author

Liang Jingqiu

Luo Hanyi

Liang Ling

Zhang Haiyan

Chen Qi

Gou Rong

朱道银

江山

Related Institution

Department of Anesthesiology， Chongqing University Cancer Hospital

Physical Examination Center，Chongqing University Cancer Hospital

重庆医科大学微生物教研室

Department of Microbiology,College of Basic Medical Science,Chongqing Medical University

重庆医科大学计生系生殖遗传教研室

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