Construction of a recombinant adenovirus vector containing HBX gene

FENG Tao; et al

doi:10.13406/j.cnki.cyxb.2004.03.006

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Construction of a recombinant adenovirus vector containing HBX gene

更新时间：2024-01-17

- Construction of a recombinant adenovirus vector containing HBX gene
- Journal of Chongqing Medical University Issue 3, Pages: 273-275(2004)
- 作者机构：
  
  1. 重庆医科大学基础医学院生物化学教研室
  2. 芝加哥大学医学中心分子肿瘤研究室
  3. 重庆医科大学基础医学院生物化学教研室,重庆,400016
  4. ,美国,60637
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2004.03.006
  CLC： R346
- Published：2004，
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[1]冯涛,何通川,宋文鑫.HBX基因重组腺病毒载体的构建[J].重庆医科大学学报,2004(03):273-275+278.

FENG Tao, et al. Construction of a recombinant adenovirus vector containing HBX gene. [J]. Journal of Chongqing Medical University (3):273-275(2004)
[1]冯涛,何通川,宋文鑫.HBX基因重组腺病毒载体的构建[J].重庆医科大学学报,2004(03):273-275+278. DOI： 10.13406/j.cnki.cyxb.2004.03.006.

FENG Tao, et al. Construction of a recombinant adenovirus vector containing HBX gene. [J]. Journal of Chongqing Medical University (3):273-275(2004) DOI： 10.13406/j.cnki.cyxb.2004.03.006.

摘要

目的 :构建携HBX基因的腺病毒载体

为进一步研究HBX基因的功能

为阐明HBV感染导致肝癌的机制研究建立基础。方法 :采用PCR技术从HBV全基因组DNA中扩增HBX基因 ;将HBX基因亚克隆至质粒 pHAHA

构建质粒 pHAHA-HBX

酶切pHAHA -HBX质粒

将HAHA -HBX片段克隆到腺病毒载体 pAd -Track -CMV中

得到 pAdTrack -CMV -HBX

同时与 pAdEasy - 1共转染 2 93细胞

确定转染效率。检测培养上清病毒中HBX的存在。结果 :将PCR扩增HBX片段成功克隆到腺病毒载体中

并经序列分析证实 ;建立了产病毒细胞株

证实重组病毒中含有HBX基因。结论 :成功构建了携HBx基因的腺病毒载体

Abstract

Objective:To establish a recombinant adenovirus vector carrying HBX gene for investigation of the functions of HBX gene on the hepatocellular carcinoma.Methods:HBX gene was amplified from genomic DNA of HBV by polymerase chain reaction(PCR) technique

and subcloned to plasmid pHAHA to construct recombinant plasmid pHAHA-HBX.subcloned HAHA-HBX into a shuttle vector pAdTrack-CMV.After being identified by endonuclease

and subsequently cotransformed into E.coli.293 cells with an adenoviral backbone plasmid

e.g.pAdEasy-1.Results:HBX gene was cloned from HBV genome by PCR successfully

and proved by sequence determination.A vector producing cell line 293/HBX was established.An HBX gene integration was detected by PCR in the recombinant adenovirus vectors.Conclusion:A recombinant adenovirus vector containing HBX gene was successfully constructed.

关键词

基因HBXHBV腺病毒载体乙型肝炎

Keywords

geneHBXHBVadenovirus vectorhepatitis B

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