Cloning and expression of survivin gene in eukaryotic cells

YAN Ge; et al

doi:10.13406/j.cnki.cyxb.2004.03.003

您当前的位置：

首页 >

文章列表页 >

Cloning and expression of survivin gene in eukaryotic cells

更新时间：2024-01-17

- Cloning and expression of survivin gene in eukaryotic cells
- Journal of Chongqing Medical University Issue 3, Pages: 266-268(2004)
- 作者机构：
  
  1. 重庆医科大学病毒性肝炎研究所
  2. Molecular Oncology Laboratory
  3. 重庆医科大学病毒性肝炎研究所,重庆,400010
  4. The University of Chicago Medical Center
  5. ,USA
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2004.03.003
  CLC： R73-3
- Published：2004，
扫描看全文
[1]闫歌,蒲丹,唐霓,张秉强,高小玲,宋文鑫,何通川,黄爱龙.凋亡抑制因子survivin基因的克隆及在真核细胞中的表达[J].重庆医科大学学报,2004(03):266-268.

YAN Ge, et al. Cloning and expression of survivin gene in eukaryotic cells. [J]. Journal of Chongqing Medical University (3):266-268(2004)
[1]闫歌,蒲丹,唐霓,张秉强,高小玲,宋文鑫,何通川,黄爱龙.凋亡抑制因子survivin基因的克隆及在真核细胞中的表达[J].重庆医科大学学报,2004(03):266-268. DOI： 10.13406/j.cnki.cyxb.2004.03.003.

YAN Ge, et al. Cloning and expression of survivin gene in eukaryotic cells. [J]. Journal of Chongqing Medical University (3):266-268(2004) DOI： 10.13406/j.cnki.cyxb.2004.03.003.

摘要

目的 :克隆Survivin编码序列并在真核细胞中表达。方法 :RT -PCR扩增Survivin编码序列

建立与绿色荧光蛋白（GFP）共表达的融合重组质粒

转染哺乳动物细胞

利用GFP绿色荧光和WesternBlot检测Survivin蛋白的表达。结果 :RT -PCR扩增出 4 2 1bp的Survivin编码序列

构建融合重组质粒pEGFP -C1-Survivin

利用GFP荧光和WesternBlot证实Sur vivin蛋白的表达。结论 :成功克隆Survivin基因并在真核细胞中表达。

Abstract

Objective:To clone and express survivin gene in mammalian cells.Methods:Coding sequence of survivin gene was amplified from HepG 2 cell mRNA by RT-PCR and then cloned into eukaryotic vector pEGFP-C1.The vector with survivin gene was transfected into HepG 2 and SMMC-7721.The expression of survivin was detected by GFP and Western blot.Results:The recombinant plasmid pEGFP-C1-survivin was successfully constructed and expressed in HepG 2 and SMMC-7721.Conclusion:The result shows expression of the anti-apoptosis gene survivin in mammalian cells.

关键词

Survivin绿色荧光蛋白真核细胞表达

Keywords

Survivingreen fluorescent proteinEukaryotic cellexpression

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Molecular mechanism of Buzhong Yiqi Decoction in coordination with Siβ-catenin in intervention against cisplatin resistance in lung adenocarcinoma by regulating the Wnt/β-catenin signaling pathway

Cloning and expression analysis of surfactant-associated protein D in cotton rat with lung injury induced by lipopolysaccharide

Activation of Wnt/β-catenin signaling pathway promotes anti-apoptotic genes bcl-2 and survivin expression in H9c2 cell subject to chronic hypoxia

Construction and expression of a recombinant hepatits c virus gene vector

The optimal electroporation condition of recombinant plasmid

Related Author

Li He

Mu Qirui

Wang Zhe

Wang Ying

Yu Dan

Gao Yuan

Feng Yi

Ren Luo

Related Institution

Department of Pathology，College of Integrative Medicine，Liaoning University of Traditional Chinese Medicine

Department of Pediatric Research Institute,the Children’s Hospital of Chongqing Medical University, Ministry of Education Key Laboratory of Child Development and Disorders,Chongqing Key Laboratory of Pediatrics,Chongqing International Science and Technology Cooperation Center for Child Development and Disorders

China International Science and Technology Cooperation Base of Child Development and Critical Disorders,Ministry of Education Key Laboratory of Child Development and Disorders,Chongqing Key Laboratory of Pediatrics

Department of Cardiology,Children’s Hospital of Chongqing Medical University

浙江医科大学传染病研究所

⁰