Effects of combination of arsenic trioxide and octreotide on proliferation and cellular apoptosis in human hepatocellular carcinoma

ZHANG Xia; et al

doi:10.13406/j.cnki.cyxb.2004.02.013

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Effects of combination of arsenic trioxide and octreotide on proliferation and cellular apoptosis in human hepatocellular carcinoma

更新时间：2024-01-17

- Effects of combination of arsenic trioxide and octreotide on proliferation and cellular apoptosis in human hepatocellular carcinoma
- Journal of Chongqing Medical University Issue 2, Pages: 162-165(2004)
- 作者机构：
  
  1. 重庆医科大学临床学院消化内科
  2. 重庆医科大学基础医学院病生教研室
  3. 重庆医科大学临床学院消化内科,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2004.02.013
  CLC： R735.7
- Published：2004，
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[1]张霞,陶小红,汤为学,谯敏.三氧化二砷与奥曲肽联用抑制人肝癌细胞株的增殖和诱导凋亡的作用[J].重庆医科大学学报,2004(02):162-165.

ZHANG Xia, et al. Effects of combination of arsenic trioxide and octreotide on proliferation and cellular apoptosis in human hepatocellular carcinoma. [J]. Journal of Chongqing Medical University (2):162-165(2004)
[1]张霞,陶小红,汤为学,谯敏.三氧化二砷与奥曲肽联用抑制人肝癌细胞株的增殖和诱导凋亡的作用[J].重庆医科大学学报,2004(02):162-165. DOI： 10.13406/j.cnki.cyxb.2004.02.013.

ZHANG Xia, et al. Effects of combination of arsenic trioxide and octreotide on proliferation and cellular apoptosis in human hepatocellular carcinoma. [J]. Journal of Chongqing Medical University (2):162-165(2004) DOI： 10.13406/j.cnki.cyxb.2004.02.013.

摘要

目的 :研究三氧化二砷（As2 O3 ）与奥曲肽（octreotide）联用对肝癌细胞生长增殖及细胞凋亡的作用

探讨其对肝癌的作用机制。方法 :应用As2 O3 和octreotide共同处理肝癌细胞株SMMC - 772 1

通过MTT法检测细胞存活率 ;用流式细胞术检测细胞周期变化及细胞凋亡率 ;透射电镜观察细胞形态变化及细胞凋亡情况。结果 :As2 O3 与octreotide联用可明显抑制SMMC - 772 1的生长

两药合用的中效浓度分别为 2 .0 2 2 1μmol/L和 8.0 88μmol/L

较两药单用时的中效浓度低（As2 O3 单用为 3.70 7μmol/L

octreotide单用为 2 2 .6 37μmol/L）。且两药合用可将肝癌细胞阻滞于S期并可诱导细胞凋亡。结论 :As2 O3 可与Octreotide协同抑制人肝癌细胞株的增殖

又能诱导其凋亡。

Abstract

Objective:To study the effects of combination of arsenic trioxide and octreotide on the proliferation and apoptosis of hepatocellular carcinoma cells(HCC)

and to explore the mechanism of their action on liver cancer.Methods:Heptocellular carcinoma cell SMMC-7721 was treated with arsenic trioxide and octreotide.Cell survival rate was determined by MTT assay;cell cycle and the apoptosis index were determined by flow cytometry (FCM);the morphological changes were observed by transmission electron microscope.Results:The combination of arsenic trioxide and octreotide significantly inhibited the growth of SMMC-7721 and the concentration of each drug in combination is 2.022μmol/L and 8.088μmol/L that was less than single use of arsenic trioxide (3.707μmol/L)and octreotide (22.637μmol/L).The combination of two drugs can arrest HCC in S phases and induce apoptosis.Conclusion:Arsenic trioxide can enhance the antiproliferative effect of octreotide to human HCC and induce apoptosis.

关键词

三氧化二砷奥曲肽联合应用增殖凋亡肝细胞癌

Keywords

arsenic trioxideoctreotideAnticancer drug combinationproliferationapoptosishepatocellular carcinoma

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Related Institution

Department of Gastrointestinal Surgery，The Third Affiliated Hospital of Zunyi Medical University/ The First People’s Hospital of Zunyi

Translational Medicine Research Center, Affiliated Hangzhou First People’s Hospital，Zhejiang University School of Medicine

Department of Gastroenterology, Affiliated Hangzhou First People’s Hospital，Zhejiang University School of Medicine

Department of Pathology,Molecular Medicine and Cancer Research Cancer,Chongqing Medical University

Department of Gynaecology and Obstetrics,The First Affiliated Hospital of Chongqing Medical University

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