Expression and  significance of  apoptosis-related  protein Fas/FasL and BcL-2 in autoimmune thyropathy

LIU Chun; et al

doi:10.13406/j.cnki.cyxb.2004.02.005

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Expression and significance of apoptosis-related protein Fas/FasL and BcL-2 in autoimmune thyropathy

更新时间：2024-01-17

- Expression and significance of apoptosis-related protein Fas/FasL and BcL-2 in autoimmune thyropathy
- Journal of Chongqing Medical University Issue 2, Pages: 134-137(2004)
- 作者机构：
  
  1. 重庆医科大学临床学院内分泌科
  2. 重庆医科大学病理学教研室,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2004.02.005
  CLC： R581
- Published：2004，
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[1]刘纯,张徽,李圆圆.凋亡相关蛋白Fas/FasL和BcL-2在自身免疫性甲状腺病中的表达及意义[J].重庆医科大学学报,2004(02):134-137.

LIU Chun, et al. Expression and significance of apoptosis-related protein Fas/FasL and BcL-2 in autoimmune thyropathy. [J]. Journal of Chongqing Medical University (2):134-137(2004)
[1]刘纯,张徽,李圆圆.凋亡相关蛋白Fas/FasL和BcL-2在自身免疫性甲状腺病中的表达及意义[J].重庆医科大学学报,2004(02):134-137. DOI： 10.13406/j.cnki.cyxb.2004.02.005.

LIU Chun, et al. Expression and significance of apoptosis-related protein Fas/FasL and BcL-2 in autoimmune thyropathy. [J]. Journal of Chongqing Medical University (2):134-137(2004) DOI： 10.13406/j.cnki.cyxb.2004.02.005.

摘要

目的 :探讨桥本氏甲状腺炎（HT）及弥漫性毒性甲状腺肿（DTG）细胞凋亡相关蛋白Fas/FasL和BcL - 2的表达及意义。方法 :采用免疫组化S -P法进行检测。结果 :HT及DTG滤泡细胞表达Fas蛋白

阳性率分别为 4 0 %和 6 0 %

正常甲状腺组织滤泡细胞则未见表达。HT及DTG分别与正常甲状腺组织相比有显著差异P <0 .0 1。在正常甲状腺组织

HT及DTG中均有滤泡细胞表达FasL和BcL - 2

而FasL的表达三者相比均无显著差异。BcL - 2的表达在正常甲状腺组织和DTG中较强

而在HT中的表达较弱

与正常甲状腺组织和DTG相比有显著差异P <0 .0 1。结论 :结果提示在HT中滤泡细胞通过表达Fas和FasL

经过Fas与FasL的作用导致部分滤泡细胞凋亡

并通过BcL - 2表达的减弱

降低了抗凋亡的作用。在DTG中由于滤泡细胞通过增强BcL - 2的表达

从而使得Fas和FasL介导的滤泡细胞凋亡作用减弱。我们认为凋亡相关蛋白的表达在自身免疫性甲状腺病的发生发展中有重要作用

Abstract

Objective:To investigate the expression of apoptosis-related protein Fas/FasL and BcL-2 and their significance in Hashimoto’s thyroiditis (HT) and diffuse toxic goiter(DTG).Methods:Immunohistochemical staining(S-P method) was used to detect expression of apoptosis-related protein in formalin-fixed and paraffin-embeded tissues from 30 cases of HT

25 cases of DTG

and 22 normal thyroid tissues.Results:Fas-positive thyrocytes were observed in HT and DTG

while expression of Fas protein in normal thyroid tissues was negative.The positive rates of Fas expression were 40% and 60% in HT and DTG respectively

compared with the normal thyroid tissues

there was a significant difference ( P <0.01).FasL-positive thyrocytes and BcL-2-positive thyrocytes were observed in HT

DTG and the normal thyroid tissues

but there was not a significant difference in intensity of FasL-positive staining ( P >0.05).Expressions of BcL-2 in normal thyroid tissues and DTG were stronger

while no significant correlation was found between them.In HT the staining of follicles for BcL-2 was very weak

and there was significant difference compared with that in the normal thyroid tissues and DTG.Conclusion:LIU Chun

et al (Department of Internal Medicine

College of Clinical Medicine

Chongqing Medical University) Abstract Objective:To investigate the expression of apoptosis-related protein Fas/FasL and BcL-2 and their significance in Hashimoto’s thyroiditis (HT) and diffuse toxic goiter(DTG).Methods:Immunohistochemical staining(S-P method) was used to detect expression of apoptosis-related protein in formalin-fixed and paraffin-embeded tissues from 30 cases of HT

25 cases of DTG

and 22 normal thyroid tissues.Results:Fas-positive thyrocytes were observed in HT and DTG

while expression of Fas protein in normal thyroid tissues was negative.The positive rates of Fas expression were 40% and 60% in HT and DTG respectively

compared with the normal thyroid tissues

there was a significant difference ( P <0.01).FasL-positive thyrocytes and BcL-2-positive thyrocytes were observed in HT

DTG and the normal thyroid tissues

but there was not a significant difference in intensity of FasL-positive staining ( P >0.05).Expressions of BcL-2 in normal thyroid tissues and DTG were stronger

while no significant correlation was found between them.In HT the staining of follicles for BcL-2 was very weak

and there was significant difference compared with that in the normal thyroid tissues and DTG.Conclusion:Our results suggest that the follicular cells in HT undergo apoptosis through expression of Fas/FasL and interaction between them.The down-regulation of BcL-2 expression in thyrocytes can decrease BcL-2-mediated suppression for apoptosis.Overexpression of BcL-2 in DTG could render thyrocytes resistant to Fas/FasL-mediated apoptosis.We conclude that the expression of apoptosis-related protein plays an important role in the pathogenesis of HT and DTG.

关键词

自身免疫性甲状腺病Fas/FaslBcl-2免疫组织化学

Keywords

Autoimmune thyropathyFas/FaslBcl-2immunohistochemistry

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